{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T20:57:23Z","timestamp":1790542643461,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4262,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1994,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objective of this study was to quantify the changes in p53 and cyclin DI protein levels in different stages of human esophageal and gastric cardia carcinogenesis in a high\u2010risk population in Henan, China. Immunoreactivity of p53, cyclin DI and proliferating\u2010cell nuclear antigen (PCNA) was observed in the cell nuclei of esophageal and gastric cardia biopsies. The number of p53\u2010immunostaining\u2010positive cells was low in normal epithelia, slightly increased in basal\u2010cell hyperplasia (BCH), markedly increased in dysplasia (DYS) (10\u2010fold), and further increased in squamous\u2010cell carcinoma (SCC) (40\u2010fold). This pattern of change was similar to that of cell proliferation as indicated by PCNA immunostaining. On the other hand, the number of cyclin DI\u2010immunostaining\u2010positive cells did not increase from BCH to DYS, although a slight increase from DYS to SCC was noted. In the gastric cardia, again, the pattern of change of p53\u2010positive cells in different stages of lesions paralleled the pattern of cell proliferation. The number of p53\u2010positive cells was very low, much lower than that of PCNA\u2010positive cells, in normal, chronic superficial gastritis (CSG) and chronic atrophic gastritis (CAG); therefore, the increase of p53\u2010positive cells from CAG to DYS was more dramatic (100\u2010fold). From DYS to adenocarcinoma (AC), the p53\u2010positive and the PCNA\u2010positive cells increased 4\u2010fold. On the other hand, the number of cyclin DI\u2010positive cells did not increase in pre\u2010cancerous lesions, but increased slightly in AC. This study demonstrates that p53 protein accumulation increased with the progression of pre\u2010cancerous lesions, especially in the genesis of dysplasia, both in the esophagus and in the gastric cardia. Our approach of quantitative immunohistochemistry sheds light on the mechanisms of genesis of esophageal and gastric\u2010cardia cancers, which frequently occur together in many highincidence areas. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910590414","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:16:31Z","timestamp":1171930591000},"page":"514-519","source":"Crossref","is-referenced-by-count":69,"title":["Changes in p<sup>53<\/sup> and cyclin D1 protein levels and cell proliferation in different stages of human esophageal and gastric\u2010cardia carcinogenesis"],"prefix":"10.1002","volume":"59","author":[{"given":"Li Dong","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie Tao","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Goldstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun\u2010Yan","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song\u2010Liang","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung S.","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"1779","article-title":"Archival analysis of p53 genetic and protein alterations in Chinese esophageal cancer","volume":"6","author":"Bennett W. 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