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Cell lines from the first group reconstituted CINIII\u2010like lesions, whereas cell lines from the second group reconstituted epithelia comparable to microinvasive carcinomas. They were thus postulated to represent distinct stages of cervical carcinogenesis. The present results have shown that lines from group I, which have conserved an epithelial morphology in monolayer, (i) could not invade matrigel when tested in a modified Boyden chamber assay, (ii) produced solely gelatinase B and (iii) were unable to activate exogenous gelatinase A. On the other hand, lines from group II associated epithelial\u2010to\u2010mesenchymal transition (acquisition of elongated morphology, vimentin positivity) with high in vitro invasive potential and with the ability both to produce and to activate gelatinase A. These results strongly support the hypothesis that the epithelial\u2010to\u2010mesenchymal transition and the associated events might be implicated in the progression to the metastatic phenotype.<\/jats:p>","DOI":"10.1002\/ijc.2910590514","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:23:52Z","timestamp":1171931032000},"page":"661-666","source":"Crossref","is-referenced-by-count":48,"title":["Epithelial\u2010to\u2010mesenchymal transition in hpv\u201033\u2010transfected cervical keratinocytes is associated with increased invasiveness and expression of gelatinase a"],"prefix":"10.1002","volume":"59","author":[{"given":"Christine","family":"Gilles","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myriam","family":"Polette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Piette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Birembaut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Michel","family":"Foidart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"3239","article-title":"A rapid in vitro assay for quantitating the invasive potential of tumor cells","volume":"47","author":"Albini A.","year":"1987","journal-title":"Cancer Res."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/85.21.1758"},{"key":"e_1_2_1_4_1","first-page":"4540","article-title":"Collagen\u2010induced activation of the M, 72,000 type\u2010IV collagenase in normal and malignant human fibroblastoid cells","volume":"52","author":"Azzam H. 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