{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:21:35Z","timestamp":1791303695503,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4246,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1994,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The glycolipids of human teratocarcinoma\u2010derived cell line NCCIT were compared with those of 5 murine teratocarcinoma\u2010derived cell lines. Glycolipid antigens were identified by cell surface immunofluorescence and high\u2010performance thin\u2010layer chromatography (HPTLC) immunostaining with a panel of monoclonal anti\u2010carbohydrate antibodies. Human NCCIT embryonal carcinoma (EC) cells contained extended globo\u2010series glycolipids Gb5 (galactosyl globoside) and GL7 (sialyl galactosyl globoside) recognized by antibodies to stage\u2010specific embryonic antigens 3 and 4 (SSEA\u20103 and \u22124). SSEA\u20104 was not detected by immunofluorescence on the surface of any of the 5 murine teratocarcinoma\u2010derived cell lines examined; however, SSEA\u20103 was detected on the surface of murine cell lines resembling primitive endoderm (JC44, NF\u2010PE) and trophectoderm (E6496D). HPTLC analysis revealed a large amount of globoside (Gb4) in these differentiated cells, which may account for their labeling with anti\u2010SSEA\u20103 antibody. Globo\u2010series glycolipids were also detected in murine EC cells; however, differences were noted between the 2 cell lines examined. F9 cells contained primarily Gb4 and Forssman glycolipid, whereas NF\u2010I cells contained only minor amounts of Gb4 and lacked Forssman glycolipid entirely. Our results, coupled with the known distribution of Forssman antigen in the egg cylinder\u2010stage mouse embryo, suggest that F9 and NF\u2010I murine EC cells are replicas of cells at different stages of development of the embryonic ectoderm. Glycolipids of normal mouse embryos were examined for comparison. Gb4 and Forssman glycolipid were present in both embryonic and extra\u2010embryonic tissues, whereas Gb5 and GL7 were restricted to visceral yolk sac and placenta. Our results demonstrate that human and murine teratocarcinoma\u2010derived cells both synthesize extended globo\u2010series glycolipids; however, oligosaccharide chain elongation takes different pathways in the 2 species. These differences reflect species\u2010related and cell type\u2010specific patterns of glycosylation.<\/jats:p>","DOI":"10.1002\/ijc.2910590518","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:23:52Z","timestamp":1171931032000},"page":"692-698","source":"Crossref","is-referenced-by-count":25,"title":["Globo\u2010series carbohydrate antigens are expressed in different forms on human and murine teratocarcinoma\u2010derived cells"],"prefix":"10.1002","volume":"59","author":[{"given":"Jason G.","family":"Krupnick","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Damjanov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Damjanov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng M.","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce A.","family":"Fenderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"11793","DOI":"10.1016\/S0021-9258(17)44300-6","article-title":"The monoclonal antibody directed to difucosylated type 2 chain (Fuc\u03b11 \u2192 2Gal\u03b21 \u2192 4\u2018Fuc\u03b11 \u2192 3\u2019 GlcNAc; Y determinant)","volume":"258","author":"Abe K.","year":"1983","journal-title":"J. biol. 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