{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T23:58:52Z","timestamp":1790985532018,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":4155,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1995,3,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Over\u2010expression of the major heat\u2010shock protein hsp70 in WEHI\u2010S tumor cells renders them resistant to the cytotoxic effects of tumor necrosis factor (TNF). To study the significance of this resistance <jats:italic>in vivo<\/jats:italic>, the tumorigenic potential of WEHI\u2010S cells transfected with human hsp70 in sense and anti\u2010sense orientation was investigated in athymic and in normal syngenic mice. A striking correlation was observed between the level of hsp70 expression and tumorigenicity in athymic mice. Hsp70 expression rendered WEHI cells tumorigenic also in normal mice, but higher numbers of cells were required for tumor formation than in athymic mice. Over\u2010expression of hsp70 in WEHI\u2010S cells did not enhance their anchorage\u2010dependent growth <jats:italic>in vitro or<\/jats:italic> their ability to form colonies in soft agar. The hsp70\u2010transfected cells exhibited greatly increased resistance against killing by murine natural cytotoxic cells and macrophages <jats:italic>in vitro.<\/jats:italic> A similar tumorigenic phenotype could also be induced independently of hsp70 by prolonged culture of WEHI\u2010S cells with TNF. These results suggest that over\u2010expression of hsp70 increases the tumorigenic potential of WEHI\u2010S cells in mice, by allowing these cells to escape from the early TNF\u2010mediated anti\u2010tumor immune surveillance.<\/jats:p>","DOI":"10.1002\/ijc.2910600520","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:38:53Z","timestamp":1171931933000},"page":"689-693","source":"Crossref","is-referenced-by-count":146,"title":["Over\u2010expression of hsp70 confers tumorigenicity to mouse fibrosarcoma cells"],"prefix":"10.1002","volume":"60","author":[{"given":"Marja","family":"J\u00e4\u00e4ttel\u00e4","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(83)90005-1"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8418500"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.9.3666"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041370119"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"957","DOI":"10.4049\/jimmunol.138.3.957","article-title":"Cell\u2010associated tumor necrosis factor (TNF) as a killing mechanism of activated cytotoxic macrophages","volume":"138","author":"Decker T.","year":"1987","journal-title":"J. Immuno."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(86)90322-4"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910510418"},{"key":"e_1_2_1_9_1","first-page":"1456","article-title":"Expression of BCL\u20102 protein enhances the survival of mouse fibrosarcoid cells in tumor\u2010necrosis\u2010factor\u2010mediated cytotoxicity","volume":"53","author":"Hennet T.","year":"1993","journal-title":"Cancer Res."},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/14.10.4127"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.8.2863"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.14.5102"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.19.6455"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"4286","DOI":"10.4049\/jimmunol.151.8.4286","article-title":"Over\u2010expression of major heat\u2010shock protein hsp70 inhibits TNF\u2010induced activation of phospholipase A2","volume":"151","author":"J\u00e4\u00e4itel\u00e4 M.","year":"1993","journal-title":"J. Immunol."},{"key":"e_1_2_1_15_1","first-page":"119","article-title":"Heat shock inhibits the cytotoxic action of TNF in tumor cells but does not alter its non\u2010cytotoxic action in adrenal and endothelial cells","volume":"10","author":"J\u00e4\u00e4ttel\u00e4 M.","year":"1991","journal-title":"Lymphokine Cytokine Res."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.3109\/07853899209149952"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.1.231"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"3507","DOI":"10.1002\/j.1460-2075.1992.tb05433.x","article-title":"Major heat\u2010shock protein hsp70 protects tumor cells from tumor\u2010necrosis\u2010factor cytotoxicity","volume":"11","author":"J\u00e4\u00e4itela M.","year":"1992","journal-title":"EMBO J."},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.3.1.1"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.1.7"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"4317","DOI":"10.4049\/jimmunol.143.12.4317","article-title":"Tumor growth in vivo selects for resistance for tumor necrosis factor","volume":"143","author":"Lattime E. C.","year":"1989","journal-title":"J. Immunol."},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ge.22.120188.003215"},{"key":"e_1_2_1_23_1","first-page":"407","volume-title":"Tumor necrosis factor. The molecules and their emerging role in medicine","author":"Malik S. T. A.","year":"1992"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.24.9517"},{"key":"e_1_2_1_25_1","first-page":"2877","article-title":"Use of polymerase chain reaction to detect the expression of the Mr\u201070,000 heat\u2010shock genes in control or heat\u2010shock leukemic cells as correlated to their heat response","volume":"50","author":"Mivechi N. F.","year":"1990","journal-title":"Cancer Res."},{"key":"e_1_2_1_26_1","doi-asserted-by":"crossref","first-page":"12424","DOI":"10.1016\/S0021-9258(18)42292-2","article-title":"The A20 zinc\u2010finger protein protects cells from TNF cytotoxicity","volume":"267","author":"Opipari A. W.","year":"1992","journal-title":"J. biol. Chem."},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/321700a0"},{"key":"e_1_2_1_28_1","doi-asserted-by":"crossref","first-page":"3457","DOI":"10.4049\/jimmunol.146.10.3457","article-title":"In vitro selection of a cell line for resistance to lysis by tumor necrosis factor\u2010\u03b1 selects for reduced tumorigenicity","volume":"146","author":"Patek P. Q.","year":"1991","journal-title":"J. Immunol."},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.174.6.1483"},{"key":"e_1_2_1_30_1","first-page":"327","article-title":"Transformation of mammalian cells to antibiotic resistance with bacterial gene under control of the SV40 early\u2010region promoter","volume":"1","author":"Southern P. J.","year":"1982","journal-title":"Mol. appl. Genet."},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.8.9.3740"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3933111"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.14.5233"},{"key":"e_1_2_1_34_1","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1128\/jvi.61.2.405-410.1987","article-title":"Medium tumor antigen of polvoma\u2010lransformation\u2010defective mutant NG59 is associated with 73\u2010kilodalton heat\u2010shock protein","volume":"61","author":"Walter G.","year":"1987","journal-title":"J. Virol."},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90944-6"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.3.629"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.18.6070"},{"key":"e_1_2_1_38_1","first-page":"803","article-title":"The gene for the rat heat\u2010shock cognate, hsc70. can suppress oncogene\u2010mediated transformation","volume":"3","author":"Yehiely F.","year":"1992","journal-title":"Cell Growth Differ."}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.2910600520","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.2910600520","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T17:57:29Z","timestamp":1698256649000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.2910600520"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,3,3]]},"references-count":37,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1995,3,3]]}},"alternative-id":["10.1002\/ijc.2910600520"],"URL":"https:\/\/doi.org\/10.1002\/ijc.2910600520","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,3,3]]}}}