{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T20:48:23Z","timestamp":1760906903206},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4141,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1995,3,16]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The \u03b1<jats:sub>5<\/jats:sub>\u03b2 integrin (CD49e\/CD29), a heterodimeric membrane protein, is the \u201cclassical\u201d fibronectin receptor on many cell types. During B\u2010cell ontogeny, expression of the \u03b1<jats:sub>5<\/jats:sub>\u2010subunit is developmentally regulated. The \u03b1\u03b2<jats:sub>1<\/jats:sub> is decisive for migration on fibronectin substrate and very likely cooperates with other adhesion molecules in transvascular trafficking. To test whether \u03b1<jats:sub>5<\/jats:sub>\u03b2 influences local growth <jats:italic>vs.<\/jats:italic> disseminative spread of neoplastic B\u2010cells <jats:italic>in vivo<\/jats:italic>, human B\u2010cell lines mimicking different maturational stages were transferred s.c. into severe combined immunodeficiency (SCID) mice and examined for \u03b1<jats:sub>5<\/jats:sub>\u03b2 expression and for adherence on fibronectin substrate <jats:italic>in vitro<\/jats:italic> and ex <jats:italic>vivo.<\/jats:italic> All cell lines were locally tumorigenic. Dissemination was observed in all animals carrying Nalm\u20106 tumors, in one animal with a BL 60 and in 2 mice carrying a Raji tumor. By contrast, Daudi, BJAB and U266 tumors did not disseminate. As evidenced by immunohistochemistry and flow cytometry, all lines and their tumors were to various extents \u03b2<jats:sub>1<\/jats:sub>\u2010positive but showed considerable differences in \u03b1<jats:sub>5<\/jats:sub> expression. The functional surface expression of \u03b1<jats:sub>5<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub>correlated with fibronectin adherence of the lines. Daudi expressed \u03b1<jats:sub>5<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub> in a non\u2010functional configuration which was rendered functional only upon applying high concentrations of Mg<jats:sup>++<\/jats:sup> and Mn<jats:sup>++<\/jats:sup>. B\u2010cell lines functionally expressing \u03b1<jats:sub>5<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub> at high or moderate levels disseminated in SCID mice while \u03b1<jats:sub>5<\/jats:sub>\u2010negative lines and Daudi did not. These results support the conclusion drawn from an earlier <jats:italic>in situ<\/jats:italic> analysis of human B\u2010cell lymphomas\/ leukemias that the \u03b1<jats:sub>5<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub> integrin contributes to the disseminative phenotype of malignant B cells.<\/jats:p>","DOI":"10.1002\/ijc.2910600623","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:38:49Z","timestamp":1171931929000},"page":"860-866","source":"Crossref","is-referenced-by-count":12,"title":["The capacity of human malignant B\u2010lymphocytes to disseminate in scid mice is correlated with functional expression of the fibronectin receptor \u03b1<sub>5<\/sub>\u03b2<sub>1<\/sub> (CD49E\/CD29)"],"prefix":"10.1002","volume":"60","author":[{"given":"Lutz","family":"Blase","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter T.","family":"Daniel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Koretz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhard","family":"Schwartz\u2010Albiez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"M\u00f6ller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"18011","DOI":"10.1016\/S0021-9258(19)84672-0","article-title":"Analysis of the role of glycosvlation of the human fibronectin receptor","volume":"264","author":"Akiyama S. 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