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Western blotting, using PKC isoenzyme\u2010specific antibodies, showed that two PKC isoenzymes, PKC \u03b2 and PKC \u03f5, were significantly decreased in cancer tissue. The level of PKC \u03b4 was increased in cancer tissue and the expression of PKC \u03b1 and \u03b6 was not altered significantly. Primary bile acids\u2014cholic acid (CA) and chenodeoxycholic acid (CDCA)\u2014and the principal secondary bile acids\u2014deoxycholic acid (DCA), lithocholic acid (LCA) and ursodeoxycholic acid (UDCA)\u2014were found to be potent and selective activators of partially purified PKC isoenzymes. PKC \u03b2 I was the isoenzyme most effectively activated by secondary bile acids. Our data provide a model for the involvement of secondary bile acids in colorectal carcinogenesis through specific PKC isoenzyme modulation in colorectal mucosa. \u00a9 <jats:italic>1995 Wiley\u2010Liss, Inc<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/ijc.2910610107","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:38:43Z","timestamp":1171931923000},"page":"35-39","source":"Crossref","is-referenced-by-count":100,"title":["Expression of protein kinase C isoenzymes in colorectal cancer tissue and their differential activation by different bile acids"],"prefix":"10.1002","volume":"61","author":[{"given":"Judit","family":"Pongracz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Penny","family":"Clark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John P.","family":"Neoptolemos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet M.","family":"Lord","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"73","article-title":"Food components and mechanisms of interest in cancer and diet in relation to their measurement","volume":"47","author":"Bingham S.","year":"1993","journal-title":"Europ. 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