{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,8]],"date-time":"2024-06-08T08:29:09Z","timestamp":1717835349541},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":4068,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1995,5,29]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Immortalized non\u2010malignant cells do not grow in soft agarose. We found, however, that HPV\u201016\u2010DNA\u2010immortalized human uterine exocervical epithelial cells (HCE 16\/3 cell line) formed colonies when co\u2010cultured with human embryonic skin fibroblasts. This appeared to be mediated by diffusible growth factors, and direct cell\u2010cell contacts were not required. HCE 16\/3 cells from colonies remained unable to grow alone in soft agarose like the parental HCE 16\/3 cells, indicating that only transient phenotypic changes without genetic alterations had occurred in the co\u2010cultures. In a modified soft\u2010agarose assay, HCE 16\/3 cells grown as an underlying monolayer did not produce enough (transforming) growth factors which could confer an anchorage\u2010independent phenotype to non\u2010transformed fibroblasts. Lethally irradiated fibroblasts were able to stimulate DNA synthesis and proliferation of HCE 16\/3 cells. Northern\u2010blot analysis showed that HPV\u201016\u2010DNA expression of the immortalized cells was not altered in the presence of fibroblasts. The expression of the IL\u2010I gene in the immortalized cells, however, was augmented by the co\u2010cultured fibroblasts compared with immortalized cells alone. In organotypic collagen raft co\u2010cultures, in which epithelial cells were grown on top of a collagen gel containing fibroblasts, HCE 16\/3 cells appeared to be chemotactic to fibroblasts and fibroblasts stimulated the growth of the immortalized epithelial cells. Our results suggest that fibroblasts may contribute to human epithelial carcinogenesis <jats:italic>in vivo.<\/jats:italic> \u00a9 <jats:italic>1995 Wiley\u2010Liss, Inc<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/ijc.2910610512","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:52:13Z","timestamp":1171932733000},"page":"658-665","source":"Crossref","is-referenced-by-count":10,"title":["Human skin fibroblasts induce anchorage\u2010independent growth of HPV\u201016\u2010DNA\u2010immortalized cervical epithelial cells"],"prefix":"10.1002","volume":"61","author":[{"given":"Jie","family":"Zheng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antti","family":"Vaheri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"3635","DOI":"10.1128\/jvi.61.11.3635-3640.1987","article-title":"The E6\u2010E7 region of human papillomavirus type 18 is sufficient for transformation of NIH 3T3 and rat\u20101 cells","volume":"61","author":"Bedell M. 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