{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:43:26Z","timestamp":1761155006256},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4031,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1995,7,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Burkitt lymphomas (BL) that arise in HLA\u2010A11\u2010positive individuals are characterized by selective loss\/down\u2010regulation of the HLA AII polypeptide. We have investigated the molecular basis of such down\u2010regulation by comparing 5 pairs of BL lines and Epstein\u2010Barr virus (EBV)\u2010transformed lymphoblastoid cell lines (LCL) derived from the normal B cells of the same individuals. The presence of apparently intact HLA A11 genes was confirmed in all 5 BL\/LCL pairs by polymerase chain reaction (PCR) typing and by Southern\u2010blot hybridization with HLA A locus\u2010specific probes. Northern\u2010blot analysis with locus\u2010and allele\u2010specific probes revealed a significantly lower expression or absence of A11\u2010specific mRNA in all 5 BL lines compared to the corresponding LCLs. Up\u2010regulation of Al1 \u2010specific mRNA was achieved by IFN\u03b1 treatment of 2 BL lines with low HLA A11 expression (BL\u201028 and BL\u201072) while the treatment had no effect in 3 BL fines (WWI \u2010BL, WW2\u2010BL and BL41) that did not express the endogenous gene. HLA A11 expression was restored by transfection of the gene in WWI\u2010BL whereas transfectants of BL\u201041 remained Al1\u2010negative. An HLA\u2010A11\u2010promoter\u2010driven chloramphenicol acetyl transferase reporter gene (pAl1 CAT) was active in WWI\u2010BL but not in BL\u201041. HLA\u2010A11 was expressed in hybrids of BL\u201041 with an A11\u2010positive LCL, while expression of the endogenous HLA A11 gene could not be restored by fusion of BL\u201041 with an A11 \u2010negative LCL, although an adequate set of transcription factors was present in the hybrid. Our results suggest that genetic defects and lack of transcription factors may contribute to the selective downregulation of HLA A11 in BL cells. \u00a9 1995 Wiley\u2010Liss Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910620117","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:49:48Z","timestamp":1171932588000},"page":"90-96","source":"Crossref","is-referenced-by-count":22,"title":["Mechanisms of allele\u2010selective down\u2010regulation of HLA class I in Burkitt's lymphoma"],"prefix":"10.1002","volume":"62","author":[{"given":"Marta P.","family":"Imreh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian\u2010Jin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro O.","family":"De Campos\u2010Lima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephan","family":"Imreh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Krausa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Browning","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria G.","family":"Masucci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910470412"},{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90509-X"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910470725"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/6.10.1485"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.7.2842"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002371-199310000-00001"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"2835","DOI":"10.4049\/jimmunol.135.4.2835","article-title":"Molecular cloning and DNA sequence analysis of genes encoding cytotoxic T lymphocyte\u2010defined HLA\u2010A3 subtypes: the El subtype","volume":"135","author":"Cowan E.","year":"1985","journal-title":"J. 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