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Seven independent cell lines established from normal human urothelium expressed complex multiple\u2010spliced CD44 mRNA transcripts when tested by RT\u2010PCR and were positive with antibodies against CD44 core epitopes and splice variants v3, v4\/5, v5 and v6. Of the 13 bladder\u2010carcinoma cell lines, all were positive for CO44 core. The more differentiated cell lines had retained some splice\u2010variant antibody reactivity and showed multiple but less complex CD44 mRNA transcript patterns, compared with normal cells. Anaplastic cell lines did not react with variant antibodies and did not contain multiple\u2010spliced CD44 transcripts. These data suggest that loss of alternatively\u2010spliced CD44 may reflect a selection pressure in the evolution of anaplastic bladder cancers. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910620415","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:46:08Z","timestamp":1171932368000},"page":"449-456","source":"Crossref","is-referenced-by-count":38,"title":["Patterns of splice variant CD44 expression by normal human urothelium <i>in situ<\/i> and <i>in vitro<\/i> and by bladder\u2010carcinoma cell lines"],"prefix":"10.1002","volume":"62","author":[{"given":"Jennifer","family":"Southgate","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludwik K.","family":"Trejdosiewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Selby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"197","article-title":"New monoclonal antibodies in CD44 and CD58: their use to quantify CD44 and CD58 on normal human erythrocytes and to compare the distribution of CD44 and CD58 in human tissues","volume":"74","author":"Anstee D. 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