{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T20:49:03Z","timestamp":1771879743526,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":3923,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1995,10,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>For quantificative determination of <jats:italic>ERBB<\/jats:italic>2 gene amplification in archival human carcinoma specimens we have developed a rapid, non\u2010radioactive approach, which is based on the differential polymerase chain reaction (PCR) and fluorescent DNA technique. Sequences from the <jats:italic>ERBB<\/jats:italic>2 gene and from a singlecopy reference gene were amplified simultaneously by PCR, in which one of each primer pair was fluorescently labelled. PCR products were separated by polyacrylamide gel electrophoresis in an automated DNA sequencer and directly quantified after laser activation and emission scanning using appropriate software. This fluorescent differential polymerase chain reaction (fd\u2010PCR) method was used for quantificative determination of <jats:italic>ERBB<\/jats:italic>2 gene amplification in 195 formalin\u2010fixed, paraffin\u2010embedded breast carcinoma tissues. <jats:italic>ERBB<\/jats:italic>2 gene amplification was found in 52 (26%) of these tumors and correlated significantly with tumor size, absence of estrogen receptor (ER) and pS2 expression, but not with absence of progesterone receptor (PR) or presence of epidermal growth factor receptor (EGF\u2010R) expression, lymph\u2010node metastases or grading. In univariate analysis, <jats:italic>ERBB<\/jats:italic>2 gene amplification showed no significant correlation with clinical outcome, either in the whole population or in the subgroup defined by positive axillary lymph\u2010node metastases. However, within the node\u2010negative subgroup, patients with <jats:italic>ERBB<\/jats:italic>2 gene amplification had significantly decreased relapse\u2010free survival and overall survival (<jats:italic>p<\/jats:italic> &lt; 0.05). The fd\u2010PCR assay is a valuable tool for determination of amplification of <jats:italic>ERBB<\/jats:italic>2 gene as well as further oncogenes. In this way, more detailed information about individual tumor biology may be acquired by a routine assay. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910640502","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T01:14:41Z","timestamp":1171934081000},"page":"291-297","source":"Crossref","is-referenced-by-count":22,"title":["<i>ERBB<\/i>2 gene amplification detected by fluorescent differential polymerase chain reaction in paraffin\u2010embedded breast carcinoma tissues"],"prefix":"10.1002","volume":"64","author":[{"given":"Han\u2010Xiang","family":"An","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dieter","family":"Niederacher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias W.","family":"Beckmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uwe\u2010Jochen","family":"G\u00f6hring","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anton","family":"Scharl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frauke","family":"Picard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudiavan","family":"van Roeyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans\u2010Georg","family":"Schn\u00fcrch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans G.","family":"Bender","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1992.10.4.599"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(05)80046-2"},{"key":"e_1_2_1_4_1","first-page":"1238","article-title":"Correlation of ERBB2 gene amplification and protein expression in human breast cancer with nodal status and nuclear grading","volume":"48","author":"Berger M. 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