{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T14:11:38Z","timestamp":1761919898940},"reference-count":49,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,11,6]],"date-time":"2014-11-06T00:00:00Z","timestamp":1415232000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Samsung Biomedical Research Institute","award":["SMX1132731"],"award-info":[{"award-number":["SMX1132731"]}]},{"name":"Samsung Biomedical Research Institute","award":["SMX1132461"],"award-info":[{"award-number":["SMX1132461"]}]},{"name":"Samsung Biomedical Research Institute","award":["OB00013"],"award-info":[{"award-number":["OB00013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2015,5,15]]},"abstract":"<jats:p>The spontaneous metastasis from human gastric carcinoma (GC) remains poorly reproduced in animal models. Here, we established an experimental mouse model in which GC progressively developed in the orthotopic stomach wall and metastasized to multiple organs; the tumors colonized in the ovary exhibited typical characteristics of Krukenberg tumor. The expression of mesenchymal markers was low in primary tumors and high in those in intravasating and extravasating veins. However, the expression of epithelial markers did not differ, indicating that the acquisition of mesenchymal markers without a concordant loss of typical epithelial markers was associated with metastasis. We identified 35 differentially expressed genes (DEGs) in GC cells metastasized to ovary, among which overexpression of <jats:italic>GAGE12<\/jats:italic> family genes, the top\u2010ranked DEGs, were validated. In addition, knockdown of the <jats:italic>GAGE12<\/jats:italic> gene family affected transcription of many of the aforementioned 35 DEGs and inhibited trans\u2010well migration, tumor sphere formation <jats:italic>in vitro and<\/jats:italic> tumor growth <jats:italic>in vivo<\/jats:italic>. In accordance, <jats:italic>GAGE12<\/jats:italic> overexpression augmented migration, tumor sphere formation and sustained <jats:italic>in vivo<\/jats:italic> tumor growth. Taken together, the <jats:italic>GAGE12<\/jats:italic> gene family promotes GC growth and metastasis by modulating the expression of GC metastasis\u2010related genes.<\/jats:p>","DOI":"10.1002\/ijc.29286","type":"journal-article","created":{"date-parts":[[2014,10,23]],"date-time":"2014-10-23T20:36:00Z","timestamp":1414096560000},"page":"2284-2292","source":"Crossref","is-referenced-by-count":16,"title":["GAGE12 mediates human gastric carcinoma growth and metastasis"],"prefix":"10.1002","volume":"136","author":[{"given":"Eun Kyung","family":"Lee","sequence":"first","affiliation":[{"name":"Samsung Biomedical Research Institute (SBRI), Samsung Medical Center and Sungkyunkwan University Seoul Korea"},{"name":"Samsung Advanced Institute for Health Sciences and Technology (SAIHST), Samsung Medical Center and Sungkyunkwan University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung\u2010A.","family":"Song","sequence":"additional","affiliation":[{"name":"Samsung Biomedical Research Institute (SBRI), Samsung Medical Center and Sungkyunkwan University Seoul Korea"},{"name":"Samsung Advanced Institute for Health Sciences and Technology (SAIHST), Samsung Medical Center and Sungkyunkwan University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u2010Hye","family":"Chae","sequence":"additional","affiliation":[{"name":"Samsung Biomedical Research Institute (SBRI), Samsung Medical Center and Sungkyunkwan University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyoung\u2010Mee","family":"Kim","sequence":"additional","affiliation":[{"name":"Samsung Biomedical Research Institute (SBRI), Samsung Medical Center and Sungkyunkwan University Seoul Korea"},{"name":"Department of Pathology and Translational Genomics Samsung Medical Center, Sungkyunkwan University School of Medicine Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seok\u2010Hyung","family":"Kim","sequence":"additional","affiliation":[{"name":"Samsung Biomedical Research Institute (SBRI), Samsung Medical Center and Sungkyunkwan University Seoul Korea"},{"name":"Samsung Advanced Institute for Health Sciences and Technology (SAIHST), Samsung Medical Center and Sungkyunkwan University Seoul Korea"},{"name":"Department of Pathology and Translational Genomics Samsung Medical Center, Sungkyunkwan University School of Medicine Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myung\u2010Soo","family":"Kang","sequence":"additional","affiliation":[{"name":"Samsung Biomedical Research Institute (SBRI), Samsung Medical Center and Sungkyunkwan University Seoul Korea"},{"name":"Samsung Advanced Institute for Health Sciences and Technology (SAIHST), Samsung Medical Center and Sungkyunkwan University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,11,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.27927"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1535-6108(04)00033-9"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-8258(92)90017-D"},{"key":"e_1_2_6_5_1","first-page":"221","article-title":"Krukenberg tumor of the ovaries","volume":"32","author":"Hale RW","year":"1968","journal-title":"A review of 81 records. 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