{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T14:57:43Z","timestamp":1793113063148,"version":"4.5.0"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,11,6]],"date-time":"2014-11-06T00:00:00Z","timestamp":1415232000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2015,5,15]]},"abstract":"<jats:p>\n                    Primary human papillomavirus (HPV)\u2010based screening results in a 2\u20135% lower specificity for cervical intraepithelial neoplasia Grade 2 or worse (CIN2+) compared to Pap cytology. To identify HPV\u2010positive women with CIN2+, we retrospectively evaluated the cross\u2010sectional and longitudinal performance of p16\/Ki\u201067 dual\u2010stained cytology in HPV\u2010positive women with normal cytology participating in population\u2010based cervical screening. Conventional Pap cytology specimens of 847 of these women derived from the VUSA\u2010Screen study were dual\u2010stained for p16\/Ki\u201067. Cross\u2010sectional clinical performance in detecting CIN3 or worse (CIN3+), and CIN2+ was compared to that of baseline HPV genotyping. Moreover, 5\u2010year cumulative incidence risks (CIR) for CIN3+ (CIN2+) were determined. The sensitivity of p16\/Ki\u201067 dual\u2010stained cytology for CIN3+ (CIN2+) was 73.3% (68.8%) with a specificity of 70.0% (72.8%). HPV16\/18 genotyping showed a sensitivity for CIN3+ (CIN2+) of 46.7% (43.8%), with a specificity of 78.3% (79.4%). The 5\u2010year CIR for CIN3+ in HPV\u2010positive women with normal cytology was 6.9%. Testing these women with p16\/Ki\u201067 dual\u2010stained cytology resulted in a significantly lower CIN3+ 5\u2010year CIR of 3.3% (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.017) in case of a negative test result. A negative HPV16\/18 genotyping test result also led to a lower 5\u2010year CIN3+ CIR of 3.6%. p16\/Ki\u201067 dual\u2010stained cytology detects more than 70% of underlying CIN3+ lesions in HPV\u2010positive women with normal cytology at baseline and is therefore suitable for triaging these women to colposcopy. Furthermore, the CIN3+ 5\u2010year CIR of 3.3% after a negative dual\u2010stain result is significantly lower compared to the 5\u2010year CIR of 6.9% in women without p16\/Ki\u201067 dual\u2010stained cytology triage.\n                  <\/jats:p>","DOI":"10.1002\/ijc.29290","type":"journal-article","created":{"date-parts":[[2014,10,25]],"date-time":"2014-10-25T05:12:58Z","timestamp":1414213978000},"page":"2361-2368","source":"Crossref","is-referenced-by-count":63,"title":["Triaging\n                    <scp>HPV<\/scp>\n                    \u2010positive women with normal cytology by p16\/\n                    <scp>K<\/scp>\n                    i\u201067 dual\u2010stained cytology testing: Baseline and longitudinal data"],"prefix":"10.1002","volume":"136","author":[{"given":"Margot H.","family":"Uijterwaal","sequence":"first","affiliation":[{"name":"Department of Pathology VU University Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole J.","family":"Polman","sequence":"additional","affiliation":[{"name":"Department of Pathology VU University Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit I.","family":"Witte","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Biostatistics VU University Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Folkert J.","family":"van Kemenade","sequence":"additional","affiliation":[{"name":"Department of Pathology Erasmus University Medical Center  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorien","family":"Rijkaart","sequence":"additional","affiliation":[{"name":"Department of Pathology VU University Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Berkhof","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Biostatistics VU University Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.A.M.A.","family":"Balfoort\u2010van der Meij","sequence":"additional","affiliation":[{"name":"Saltro Diagnostic Centre PO BOX 9300 3506 GH Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruediger","family":"Ridder","sequence":"additional","affiliation":[{"name":"Ventana Medical Systems, Inc. 1910 E Innovation Park Drive Tucson, AZ 85755 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.F.","family":"Snijders","sequence":"additional","affiliation":[{"name":"Department of Pathology VU University Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris J.L.M.","family":"Meijer","sequence":"additional","affiliation":[{"name":"Department of Pathology VU University Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,11,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21955"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.06.095"},{"key":"e_1_2_6_4_1","unstructured":"Standpunt minister van VWS screening op baarmoederhalskanker. Available from:www.rivm.nl\/Documenten_en_publicaties\/Algemeen_Actueel\/Uitgaven\/Preventie_Ziekte_Zorg\/baarmoederhalskankerscreening\/Standpunt_minister_van_VWS_screening_op_baarmoederhalskanker(accessed on January 31 2014)."},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djn065"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/lgt.0b013e31815ea58b"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.26056"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-13-0173"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24891"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2011.02.033"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(12)70529-6"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djt235"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2011.581"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2003.011841"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000326965"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.3.779-787.2002"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.2000.tb13299.x"},{"key":"e_1_2_6_18_1","first-page":"292","volume-title":"Obstetrical and gynaecological pathology","author":"Anderson MC","year":"2013"},{"key":"e_1_2_6_19_1","first-page":"19","article-title":"Pathology databanking and biobanking in The Netherlands, a central role for PALGA, the nationwide histopathology and cytopathology data network and archive","volume":"29","author":"Casparie M","year":"2007","journal-title":"Cell Oncol"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20076"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2012.5"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0006-341X.2000.00345.x"},{"key":"e_1_2_6_23_1","volume-title":"R: a language and environment for statistical Computing","author":"R Development Core team","year":"2013"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14955-0"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.981"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2303.2002.00394.x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0895-4356(92)90129-B"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197810262991705"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.29290","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.29290","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T13:14:17Z","timestamp":1696338857000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.29290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11,6]]},"references-count":27,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2015,5,15]]}},"alternative-id":["10.1002\/ijc.29290"],"URL":"https:\/\/doi.org\/10.1002\/ijc.29290","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11,6]]}}}