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Improved understanding of the cellular events during androgen deprivation would help to identify survival and stress pathways whose inhibition could synergize with androgen deprivation. Toward this aim, we performed an RNAi screen on 2,068 genes, including kinases, phosphatases, epigenetic enzymes and other druggable gene targets. High\u2010content cell spot microarray (CSMA) screen was performed in VCaP cells in the presence and absence of androgens with detection of Ki67 and cleaved ADP\u2010ribose polymerase (cPARP) as assays for cell proliferation and apoptosis. Thirty\u2010nine candidate genes were identified, whose silencing inhibited proliferation or induced apoptosis of VCaP cells exclusively under androgen\u2010deprived conditions. One of the candidates, HSPB (heat shock 27 kDa)\u2010associated protein 1 (HSPBAP1), was confirmed to be highly expressed in tumor samples and its mRNA expression levels increased with the Gleason grade. We found that strong HSPBAP1 immunohistochemical staining (IHC) was associated with shorter disease\u2010specific survival of PCa patients compared with negative to moderate staining. Furthermore, we demonstrate that HSPBAP1 interacts with AR in the nucleus of PCa cells specifically during androgen\u2010deprived conditions, occupies chromatin at <jats:italic>PSA\/klk3<\/jats:italic> and <jats:italic>TMPRSS2\/tmprss2<\/jats:italic> enhancers and regulates their expression. In conclusion, we suggest that HSPBAP1 aids in sustaining cell viability by maintaining AR signaling during androgen\u2010deprived conditions.<\/jats:p>","DOI":"10.1002\/ijc.29303","type":"journal-article","created":{"date-parts":[[2014,10,30]],"date-time":"2014-10-30T17:20:11Z","timestamp":1414689611000},"page":"2535-2545","source":"Crossref","is-referenced-by-count":9,"title":["Androgen receptor\u2010interacting protein <scp>HSPBAP1<\/scp> facilitates growth of prostate cancer cells in androgen\u2010deficient conditions"],"prefix":"10.1002","volume":"136","author":[{"given":"Khalid","family":"Saeed","sequence":"first","affiliation":[{"name":"Institute for Molecular Medicine Finland, FIMM, University of Helsinki Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P\u00e4ivi","family":"\u00d6stling","sequence":"additional","affiliation":[{"name":"Institute for Molecular Medicine Finland, FIMM, University of Helsinki Helsinki 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