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The implication of M\u2010CSF in oncology was initially suspected by the reduced metastatic dissemination in knock\u2010out mice, due to angiogenesis impairment. Based on this observation, our work studied the involvement of IL\u201034 in the pathogenesis of osteosarcoma. The<jats:italic>in vivo<\/jats:italic>effects of IL\u201034 were assessed on tissue vasculature and macrophage infiltration in a murine preclinical model based on a paratibial inoculation of human osteosarcoma cells overexpressing or not IL\u201034 or M\u2010CSF.<jats:italic>In vitro<\/jats:italic>investigations using endothelial cell precursors and mature HUVEC cells were performed to analyse the involvement of IL\u201034 in angiogenesis and myeloid cell adhesion. The data revealed that IL\u201034 overexpression was associated with the progression of osteosarcoma (tumor growth, lung metastases) and an increase of neo\u2010angiogenesis.<jats:italic>In vitro<\/jats:italic>analyses demonstrated that IL\u201034 stimulated endothelial cell proliferation and vascular cord formation. Pre\u2010treatment of endothelial cells by chondroitinases\/heparinases reduced the formation of vascular tubes and abolished the associated cell signalling. In addition, IL\u201034 increased the<jats:italic>in vivo<\/jats:italic>recruitment of M2 tumor\u2010associated macrophages into the tumor tissue. IL\u201034 increased<jats:italic>in vitro<\/jats:italic>monocyte\/CD34<jats:sup>+<\/jats:sup>cell adhesion to activated HUVEC monolayers under physiological shear stress conditions. This work also demonstrates that IL\u201034 is expressed by osteosarcoma cells, is regulated by TNF\u2010\u03b1, IL\u20101\u03b2, and contributes to osteosarcoma growth by increasing the neo\u2010angiogenesis and the recruitment of M2 macrophages. By promoting new vessel formation and extravasation of immune cells, IL\u201034 may play a key role in tumor development and inflammatory diseases.<\/jats:p>","DOI":"10.1002\/ijc.29376","type":"journal-article","created":{"date-parts":[[2014,12,5]],"date-time":"2014-12-05T03:09:59Z","timestamp":1417748999000},"page":"73-85","source":"Crossref","is-referenced-by-count":179,"title":["Interleukin\u201034 promotes tumor progression and metastatic process in osteosarcoma through induction of angiogenesis and macrophage recruitment"],"prefix":"10.1002","volume":"137","author":[{"given":"Aude I.","family":"S\u00e9galiny","sequence":"first","affiliation":[{"name":"INSERM, UMR 957, Equipe LIGUE Nationale Contre le Cancer 2012 Nantes 44035 France"},{"name":"Universit\u00e9 de Nantes, Nantes atlantique universit\u00e9s Pathophysiology of Bone Resorption and Therapy of Primary Bone Tumours Nantes 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