{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:34:27Z","timestamp":1784334867079,"version":"3.55.0"},"reference-count":47,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,8,18]],"date-time":"2015-08-18T00:00:00Z","timestamp":1439856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["R01 CA154460-01"],"award-info":[{"award-number":["R01 CA154460-01"]}]},{"name":"NIH","award":["U01 CA176067-01A1"],"award-info":[{"award-number":["U01 CA176067-01A1"]}]},{"name":"NIH","award":["CA-16359"],"award-info":[{"award-number":["CA-16359"]}]},{"name":"Deborah Bunn Alley Foundation, the Tina Brozman Foundation, the Discovery to Cure Foundation, Guido Berlucchi Foundation"},{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"crossref","award":["RF-2010-2313497"],"award-info":[{"award-number":["RF-2010-2313497"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>Identification of micrometastatic disease at the time of surgery remains extremely challenging in ovarian cancer patients. We used fluorescence microscopy, an <jats:italic>in vivo<\/jats:italic> imaging system and a fluorescence stereo microscope to evaluate fluorescence distribution in Claudin\u20103\u2010 and \u20104\u2010overexpressing ovarian tumors, floating tumor clumps isolated from ascites and healthy organs. To do so, mice harboring chemotherapy\u2010na\u00efve and chemotherapy\u2010resistant human ovarian cancer xenografts or patient\u2010derived xenografts (PDXs) were treated with the carboxyl\u2010terminal binding domain of the <jats:italic>Clostridium perfringens<\/jats:italic> enterotoxin (c\u2010CPE) conjugated to FITC (FITC\u2010c\u2010CPE) or the near\u2010infrared (NIR) fluorescent tag IRDye CW800 (CW800\u2010c\u2010CPE) either intraperitoneally (IP) or intravenously (IV). We found tumor fluorescence to plateau at 30 min after IP injection of both the FITC\u2010c\u2010CPE and the CW800\u2010c\u2010CPE peptides and to be significantly higher than in healthy organs (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). After IV injection of CW800\u2010c\u2010CPE, tumor fluorescence plateaued at 6 hr while the most favorable tumor\u2010to\u2010background fluorescence ratio (TBR) was found at 48 hr in both mouse models. Importantly, fluorescent c\u2010CPE was highly sensitive for the <jats:italic>in vivo<\/jats:italic> visualization of peritoneal micrometastatic tumor implants and the identification of ovarian tumor spheroids floating in malignant ascites that were otherwise not detectable by conventional visual observation. The use of the fluorescent c\u2010CPE peptide may represent a novel and effective optical approach at the time of primary debulking surgery for the real\u2010time detection of micrometastatic ovarian disease overexpressing the Claudin\u20103 and \u20104 receptors or the identification of residual disease at the time of interval debulking surgery after neoadjuvant chemotherapy treatment.<\/jats:p>","DOI":"10.1002\/ijc.29632","type":"journal-article","created":{"date-parts":[[2015,6,11]],"date-time":"2015-06-11T01:29:10Z","timestamp":1433986150000},"page":"2618-2629","source":"Crossref","is-referenced-by-count":28,"title":["<i>Clostridium perfringens<\/i> enterotoxin <scp>C<\/scp>\u2010terminal domain labeled to fluorescent dyes for <i>in vivo<\/i> visualization of micrometastatic chemotherapy\u2010resistant ovarian cancer"],"prefix":"10.1002","volume":"137","author":[{"given":"Emiliano","family":"Cocco","sequence":"first","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences Yale School of Medicine New Haven CT"},{"name":"Department of Molecular and Translational Medicine Palazzetto Polifunzionale Brescia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik M.","family":"Shapiro","sequence":"additional","affiliation":[{"name":"Department of Radiology Michigan State University East Lansing MI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Gasparrini","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences Yale School of Medicine New Haven CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Lopez","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences Yale School of Medicine New Haven CT"},{"name":"Division of Gynecologic Oncology University Campus Bio\u2010Medico of Rome Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlton L.","family":"Schwab","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences Yale School of Medicine New Haven CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Bellone","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences Yale School of Medicine New Haven CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ileana","family":"Bortolomai","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences Yale School of Medicine New Haven CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia J.","family":"Sumi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences Yale School of Medicine New Haven CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Bonazzoli","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences Yale School of Medicine New Haven CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Nicoletti","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences Yale School of Medicine New Haven CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Yale University New Haven CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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