{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T21:09:37Z","timestamp":1792271377406,"version":"4.4.0"},"reference-count":21,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Institut National du Cancer (INCa), SPLIT project","award":["2011\/196"],"award-info":[{"award-number":["2011\/196"]}]},{"DOI":"10.13039\/100007391","name":"Association pour la Recherche sur le Cancer (ARC)","doi-asserted-by":"crossref","award":["20111204169"],"award-info":[{"award-number":["20111204169"]}],"id":[{"id":"10.13039\/100007391","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100013501","name":"INCA","doi-asserted-by":"crossref","award":["2014\/122"],"award-info":[{"award-number":["2014\/122"]}],"id":[{"id":"10.13039\/100013501","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2015,12,15]]},"abstract":"<jats:p>A fraction of oropharyngeal cancer (OPC), especially in the tonsil, is caused by human papillomavirus (HPV), mainly HPV16. Noninvasive diagnostic methods to detect precancerous lesions in the tonsil would be useful, <jats:italic>e.g<\/jats:italic>., liquid\u2010based cytology (LBC). However, ill\u2010characterized precancerous lesions may be hidden in the depth of the tonsillar crypts. We therefore conducted a study on HPV and tonsillar precancerous lesions to evaluate, among other things, the utility of LBC obtained by deep brushing of the resected tonsils. Two hundred non\u2010paediatric patients (mean age: 30.3 years) who underwent tonsillectomy for infection\u2010related conditions (69%) or other conditions (mainly obstructive sleep apnoea, 31%) were included. An ultra\u2010sensitive Luminex bead\u2010based platform was used to test for the DNA of 21 mucosal HPV types; 56% of slides were unsatisfactory due to low number of squamous epithelial cells or the masking effect of a large number of lymphocytes. Three patients (1.5%; 95% CI: 0.5\u20134.3) showed suspicious cytological findings (atypical squamous cells\u2010cannot exclude high\u2010grade squamous intraepithelial lesion, ASC\u2010H) while 3 others were HPV\u2010positive (2 for HPV16 and 1 for HPV39). None of the ASC\u2010H patients and HPV\u2010positive patients showed dysplasia at histological examination. The rarity of HPV infection in the tonsil conflicts with the relatively frequent detection of the virus in the mouth. In conclusion, aggressive deep brushing of tonsils, while hardly applicable <jats:italic>in vivo<\/jats:italic>, is unlikely to be a reliable method to detect precancerous lesions. The absence of OPC screening modalities places the priority on multi\u2010purpose primary prevention strategies, <jats:italic>i.e<\/jats:italic>., HPV vaccination and reduction of smoking and drinking.<\/jats:p>","DOI":"10.1002\/ijc.29660","type":"journal-article","created":{"date-parts":[[2015,6,26]],"date-time":"2015-06-26T08:33:12Z","timestamp":1435307592000},"page":"2994-2999","source":"Crossref","is-referenced-by-count":23,"title":["Deep brush\u2010based cytology in tonsils resected for benign diseases"],"prefix":"10.1002","volume":"137","author":[{"given":"Silvia","family":"Franceschi","sequence":"first","affiliation":[{"name":"International Agency for Research on Cancer 69372 Lyon cedex 08 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Damien","family":"Combes","sequence":"additional","affiliation":[{"name":"International Agency for Research on Cancer 69372 Lyon cedex 08 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Dalstein","sequence":"additional","affiliation":[{"name":"CHU Reims H\u00f4pital Maison Blanche Laboratoire Pol Bouin Histologie \u2010 Cytologie \u2010 Biologie Cellulaire Reims, F\u201051092 France"},{"name":"INSERM UMR\u2010S 903 Reims F\u201051092 France Universit\u00e9 de Reims Champagne\u2010Ardenne, Facult\u00e9 de M\u00e9decine Reims F\u201051095 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Caudroy","sequence":"additional","affiliation":[{"name":"CHU Reims H\u00f4pital Maison Blanche Laboratoire Pol Bouin Histologie \u2010 Cytologie \u2010 Biologie Cellulaire Reims, F\u201051092 France"},{"name":"INSERM UMR\u2010S 903 Reims F\u201051092 France Universit\u00e9 de Reims Champagne\u2010Ardenne, Facult\u00e9 de M\u00e9decine Reims F\u201051095 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Clifford","sequence":"additional","affiliation":[{"name":"International Agency for Research on Cancer 69372 Lyon cedex 08 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarik","family":"Gheit","sequence":"additional","affiliation":[{"name":"International Agency for Research on Cancer 69372 Lyon cedex 08 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Tommasino","sequence":"additional","affiliation":[{"name":"International Agency for Research on Cancer 69372 Lyon cedex 08 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Clavel","sequence":"additional","affiliation":[{"name":"CHU Reims H\u00f4pital Maison Blanche Laboratoire Pol Bouin Histologie \u2010 Cytologie \u2010 Biologie Cellulaire Reims, F\u201051092 France"},{"name":"INSERM UMR\u2010S 903 Reims F\u201051092 France Universit\u00e9 de Reims Champagne\u2010Ardenne, Facult\u00e9 de M\u00e9decine Reims F\u201051095 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Lacau St Guily","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology and Head and Neck Surgery Faculty of Medicine\u2010Pierre Et Marie Curie University\u2010Paris VI and Tenon Hospital, Assistance Publique H\u00f4pitaux De Paris  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Birembaut","sequence":"additional","affiliation":[{"name":"CHU Reims H\u00f4pital Maison Blanche Laboratoire Pol Bouin Histologie \u2010 Cytologie \u2010 Biologie Cellulaire Reims, F\u201051092 France"},{"name":"INSERM UMR\u2010S 903 Reims F\u201051092 France Universit\u00e9 de Reims Champagne\u2010Ardenne, Facult\u00e9 de M\u00e9decine Reims F\u201051095 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"for the Study of Natural History of Human Papillomavirus Infection and Precancerous Lesions in the Tonsils (SPLIT)","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,14]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"IARC. 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