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S100P is a member of S100 family proteins and plays important roles in cancers. We investigated the S100P expression and its correlation with clinicopathology in 78 cases of opisthorchiasis\u2010associated CCA, and the effects of S100P knockdown with shRNA interference on the proliferation, cell cycle, migration, apoptosis and sensitivity to anti\u2010cancer drug. Extremely high expression of S100P mRNA was detected in the CCA tumor tissues. The increased S100P protein expression was immunohistochemically confirmed and localized in the CCA cytoplasm and\/or nuclei as well as in the hyperneoplasia and dysplasia bile ducts, but not in normal bile ducts. The intensity of immunostaining was correlated with survival, tumor stage and metastasis, and the high expression could be an independent prognostic factor. High levels of S100P were detected in the serum and bile fluid of CCA patients. The shRNA\u2010mediated knockdown of S100P expression inhibited the proliferation <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>, and migration of CCA cells, arrested cell cycle with the up\u2010regulated expression of cell cycle arrest related factors, p21, p27, GADD45A, and 14\u20103\u20103 zeta. S100P knockdown also promoted CCA cell apoptosis by up\u2010regulating expression of apoptosis related factors, DR5, TRADD, caspase 3 and BAX, and increased the sensitivity of CCA cells to the chemotherapeutic agents sunitinib and apigenin. Taken together, this study indicates that S100P might be a promising biomarker for the diagnosis, prognosis and therapy of CCA.<\/jats:p>","DOI":"10.1002\/ijc.29721","type":"journal-article","created":{"date-parts":[[2015,8,8]],"date-time":"2015-08-08T15:35:29Z","timestamp":1439048129000},"page":"396-408","source":"Crossref","is-referenced-by-count":40,"title":["Significance of <scp>S100P<\/scp> as a biomarker in diagnosis, prognosis and therapy of opisthorchiasis\u2010associated cholangiocarcinoma"],"prefix":"10.1002","volume":"138","author":[{"given":"Zhiliang","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Parasitology and Infectious Diseases Gifu University Graduate School of Medicine  Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thidarut","family":"Boonmars","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine Khon Kaen University, Khon Kaen  Thailand"},{"name":"Liver Fluke and 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