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Activation of NF\u2010\u03baB is common in NPC and may contribute to NPC development. The role of NF\u2010\u03baB activation in immortalization of nasopharyngeal epithelial (NPE) cells, which may represent an early event in NPC pathogenesis, is unknown. Examination of NF\u2010\u03baB activation in immortalization of NPE cells is of particular interest as the site of NPC is often heavily infiltrated with inflammatory cellular components. We found that constitutive activation of NF\u2010\u03baB signaling is a common phenotype in telomerase\u2010immortalized NPE cell lines. Our results suggest that NF\u2010\u03baB activation promotes the growth of telomerase\u2010immortalized NPE cells, and suppression of NF\u2010\u03baB activity inhibits their proliferation. Furthermore, we observed upregulation of c\u2010Myc, IL\u20106 and Bmi\u20101 in our immortalized NPE cells. Inhibition of NF\u2010\u03baB downregulated expression of c\u2010Myc, IL\u20106 and Bmi\u20101, suggesting that they are downstream events of NF\u2010\u03baB activation in immortalized NPE cells. We further delineated that EGFR\/MEK\/ERK\/IKK\/mTORC1 is the key upstream pathway of NF\u2010\u03baB activation in immortalized NPE cells. Elucidation of events underlying immortalization of NPE cells may provide insights into early events in pathogenesis of NPC. The identification of NF\u2010\u03baB activation and elucidation of its activation mechanism in immortalized NPE cells may reveal novel therapeutic targets for treatment and prevention of NPC.<\/jats:p>","DOI":"10.1002\/ijc.29850","type":"journal-article","created":{"date-parts":[[2015,9,15]],"date-time":"2015-09-15T07:35:52Z","timestamp":1442302552000},"page":"1175-1185","source":"Crossref","is-referenced-by-count":36,"title":["Significance of <scp>NF\u2010\u03baB<\/scp> activation in immortalization of nasopharyngeal epithelial cells"],"prefix":"10.1002","volume":"138","author":[{"given":"Dan Dan","family":"Zhu","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences and Center for Cancer Research, Li Ka Shing Faculty of Medicine The University of Hong Kong  Hong Kong, SAR, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences and Center for Cancer 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