{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T10:26:18Z","timestamp":1790331978807,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,2,8]],"date-time":"2016-02-08T00:00:00Z","timestamp":1454889600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["5\/4\/3\u20138\/09\u2010NCD\u2010II"],"award-info":[{"award-number":["5\/4\/3\u20138\/09\u2010NCD\u2010II"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:p>Controversies about the origin of circulating miRNAs have encouraged us to identify organ specific circulating miRNAs as disease biomarkers. To identify liver\u2010specific miRNAs for hepatocellular carcinoma (HCC), global expression profiling of miRNAs in liver tissue of HBV\u2010HCC and HBV\u2010control with no or mild fibrosis was evaluated. A total of 40 differentially expressed miRNAs were identified in HCC. Among ten highly altered miRNAs, six miRNAs were successfully validated in tissues, whereas only two miRNAs, miR\u2010126 and miR\u2010142\u20103p showed increased expression in plasma of HBV\u2010HCC compared to HBV\u2010non\u2010HCC patients. Subsequently, ROC curve analysis revealed that neither miR\u2010126 nor miR\u2010142\u20103p performed better than AFP in discriminating HCC from non\u2010HCC while combination of each with AFP showed significantly higher efficiency rather than AFP alone (AUC: 0.922, 0.908 <jats:italic>vs<\/jats:italic>. 0.88; sensitivity: 0.84, 0.86 <jats:italic>vs<\/jats:italic>. 0.82 and specificity: 0.92, 0.94 <jats:italic>vs<\/jats:italic>. 0.86 respectively). Interestingly, triple combination of markers (miR\u2010126\u2009+\u2009miR\u2010142\u20103p\u2009+\u2009AFP) showed no additive effect on efficiency (AUC: 0.925) over the dual combination. Again, the expression of only miR\u2010126 was noticed significantly higher in HBV\u2010HCC patients with low\u2010AFP [&lt;250 ng\/ml] compared to either non\u2010HCC or liver cirrhosis (AUC: 0.77, 0.64, respectively). Furthermore, no alteration in expression of mir\u2010126 in HCV\u2010HCC or non\u2010viral\u2010HCC revealed that miR\u2010126\u2009+\u2009AFP might be specific to HBV\u2010HCC. To understand the physiological role of these two miRNAs in hepato\u2010carcinogenesis, target genes related to cancer pathways (APAF1, APC2, CDKN2A, IRS1, CRKL, LIFR, EGR2) were verified. Thus, combination of circulating miR\u2010126\u2009+\u2009AFP is a promising noninvasive diagnostic biomarker for HBV\u2010HCC and may be useful in the management of HCC patients.<\/jats:p>","DOI":"10.1002\/ijc.29999","type":"journal-article","created":{"date-parts":[[2016,1,13]],"date-time":"2016-01-13T02:40:21Z","timestamp":1452652821000},"page":"2732-2744","source":"Crossref","is-referenced-by-count":47,"title":["Hepatic mi<scp>R<\/scp>\u2010126 is a potential plasma biomarker for detection of hepatitis <scp>B<\/scp> virus infected hepatocellular carcinoma"],"prefix":"10.1002","volume":"138","author":[{"given":"Amit","family":"Ghosh","sequence":"first","affiliation":[{"name":"Department of Hepatology and School of Digestive and Liver Diseases Centre for Liver Research, Institute of Post Graduate Medical Education and Research Kolkata 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