{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T13:24:20Z","timestamp":1790429060552,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,2,8]],"date-time":"2016-02-08T00:00:00Z","timestamp":1454889600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000289","name":"Cancer Research UK","doi-asserted-by":"crossref","award":["C569\/A10404"],"award-info":[{"award-number":["C569\/A10404"]}],"id":[{"id":"10.13039\/501100000289","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:p>High\u2010risk human papillomavirus (hrHPV) DNA tests have excellent sensitivity for detection of cervical intraepithelial neoplasia 2 or higher (CIN2+). A drawback of hrHPV screening, however, is modest specificity. Therefore, hrHPV\u2010positive women might need triage to reduce adverse events and costs associated with unnecessary colposcopy. We compared the performance of HPV16\/18 genotyping with a predefined DNA methylation triage test (S5) based on target regions of the human gene <jats:italic>EPB41L3<\/jats:italic>, and viral late gene regions of HPV16, HPV18, HPV31 and HPV33. Assays were run using exfoliated cervical specimens from 710 women attending routine screening, of whom 38 were diagnosed with CIN2+ within a year after triage to colposcopy based on cytology and 341 were hrHPV positive. Sensitivity and specificity of the investigated triage methods were compared by McNemar's test. At the predefined cutoff, S5 showed better sensitivity than HPV16\/18 genotyping (74% vs 54%, <jats:italic>P<\/jats:italic>\u2009=\u20090.04) in identifying CIN2+ in hrHPV\u2010positive women, and similar specificity (65% vs 71%, <jats:italic>P<\/jats:italic>\u2009=\u20090.07). When the S5 cutoff was altered to allow equal sensitivity to that of genotyping, a significantly higher specificity of 91% was reached (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001). Thus, a DNA methylation test for the triage of hrHPV\u2010positive women on original screening specimens might be a valid approach with better performance than genotyping.<\/jats:p>","DOI":"10.1002\/ijc.30008","type":"journal-article","created":{"date-parts":[[2016,1,20]],"date-time":"2016-01-20T23:06:46Z","timestamp":1453331206000},"page":"2745-2751","source":"Crossref","is-referenced-by-count":95,"title":["Validation of a <scp>DNA<\/scp> methylation <scp>HPV<\/scp> triage classifier in a screening sample"],"prefix":"10.1002","volume":"138","author":[{"given":"Attila T.","family":"Lorincz","sequence":"first","affiliation":[{"name":"Queen Mary University of London, Centre for Cancer Prevention, Wolfson Institute of Preventive Medicine, Barts and the London School of Medicine Charterhouse Square London EC1M 6BQ United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam R.","family":"Brentnall","sequence":"additional","affiliation":[{"name":"Queen Mary 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