{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T04:24:36Z","timestamp":1696393476494},"reference-count":51,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,4,26]],"date-time":"2016-04-26T00:00:00Z","timestamp":1461628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Danish Cancer Society and the Mermaid II Project"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2016,10,15]]},"abstract":"<jats:p>In this prospective cohort study, we compared the performance of human papillomavirus (HPV) mRNA and DNA testing of women with atypical squamous cells of undetermined significance (ASC\u2010US) during cervical cancer screening. Using a nationwide Danish pathology register, we identified women aged 30\u201365 years with ASC\u2010US during 2005\u20132011 who were tested for HPV16\/18\/31\/33\/45 mRNA using PreTect HPV\u2010Proofer (<jats:italic>n<\/jats:italic>\u2009=\u20093,226) or for high\u2010risk HPV (hrHPV) DNA using Hybrid Capture 2 (HC2) (<jats:italic>n<\/jats:italic>\u2009=\u20099,405) or Linear Array HPV\u2010Genotyping test (LA) (<jats:italic>n<\/jats:italic>\u2009=\u20091,533). Women with \u22651 subsequent examination in the register (<jats:italic>n<\/jats:italic>\u2009=\u200913,729) were followed for up to 9.5 years for high\u2010grade cervical intraepithelial neoplasia (CIN) or cancer. After 3 years' follow\u2010up, mRNA testing had higher specificity for CIN3 or worse (CIN3+) than HC2 testing (88.1% [95% confidence interval (CI): 86.8\u201389.6%] <jats:italic>versus<\/jats:italic> 59.3% [95% CI: 58.1\u201360.4%]) and higher positive predictive value (PPV) (38.2% [95% CI: 33.8%\u201343.1%] <jats:italic>versus<\/jats:italic> 19.5% [95% CI: 17.8\u201320.9%]). However, the sensitivity of mRNA testing was lower than that of HC2 testing (66.7% [95% CI: 59.3\u201374.5%] <jats:italic>versus<\/jats:italic> 97.0% [95% CI: 95.5\u201398.4%]), and women testing mRNA negative had higher 3\u2010year risk for CIN3+ than those testing HC2 negative (3.2% [95% CI: 2.2\u20134.2%] <jats:italic>versus<\/jats:italic> 0.5% [95% CI: 0.3\u20130.7%]). Patterns were similar after 18 months and 5 years'; follow\u2010up; for CIN2+ and cancer as outcomes; across all age groups; and when comparing mRNA testing to hrHPV DNA testing using LA. In conclusion, the HPV16\/18\/31\/33\/45 mRNA test is not optimal for ASC\u2010US triage due to its low sensitivity and the substantial risk for precancer following a negative test.<\/jats:p>","DOI":"10.1002\/ijc.30104","type":"journal-article","created":{"date-parts":[[2016,3,23]],"date-time":"2016-03-23T08:37:26Z","timestamp":1458722246000},"page":"1839-1850","source":"Crossref","is-referenced-by-count":2,"title":["Human papillomavirus mRNA and DNA testing in women with atypical squamous cells of undetermined significance: A prospective cohort study"],"prefix":"10.1002","volume":"139","author":[{"given":"Louise T.","family":"Thomsen","sequence":"first","affiliation":[{"name":"Unit of Virus, Lifestyle and Genes, Danish Cancer Society Research Center, Danish Cancer Society Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Dehlendorff","sequence":"additional","affiliation":[{"name":"Unit of Statistics, Bioinformatics and Registry, Danish Cancer Society Research Center Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jette","family":"Junge","sequence":"additional","affiliation":[{"name":"Department of Pathology Hvidovre Hospital, University of Copenhagen Hvidovre Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marianne","family":"Waldstr\u00f8m","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology Vejle Hospital Vejle Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doris","family":"Schledermann","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology Odense University Hospital Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirsten","family":"Frederiksen","sequence":"additional","affiliation":[{"name":"Unit of Statistics, Bioinformatics and Registry, Danish Cancer Society Research Center Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne K.","family":"Kjaer","sequence":"additional","affiliation":[{"name":"Unit of Virus, Lifestyle and Genes, Danish Cancer Society Research Center, Danish Cancer Society Copenhagen Denmark"},{"name":"Department of Gynecology Rigshospitalet University Hospital Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,4,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2014.362"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2013.04.024"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(11)70145-0"},{"key":"e_1_2_6_5_1","unstructured":"Dansk Kvalitetsdatabase for Livmoderhalskr\u00e6ftscreening [in Danish] [Danish Quality Assurance Database for Cervical Cancer Screening]. \u00c5rsrapport2014[Annual report 2014]. 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