{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T21:04:27Z","timestamp":1758402267762},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,6,24]],"date-time":"2016-06-24T00:00:00Z","timestamp":1466726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"University of Malaya, Malaysia (UM-MOHE High Impact Research Grant)","award":["UM.C\/625\/1\/1HIR\/282 MOHE\/CHAN-02(H-50001-A000023)"],"award-info":[{"award-number":["UM.C\/625\/1\/1HIR\/282 MOHE\/CHAN-02(H-50001-A000023)"]}]},{"name":"Ministry of Health, Malaysia","award":["(4)dlm.KKM\/NIHSEC\/08\/0804\/MRG-IMR","(64)KKM\/NIHSEC\/08\/0804-02 Jld2 (64))","NMRR-12-1203-14027"],"award-info":[{"award-number":["(4)dlm.KKM\/NIHSEC\/08\/0804\/MRG-IMR","(64)KKM\/NIHSEC\/08\/0804-02 Jld2 (64))","NMRR-12-1203-14027"]}]},{"name":"Ion Torrent\u2122 Transcriptome Profiling Grant Program"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2016,10,15]]},"abstract":"<jats:p>Nasopharyngeal carcinoma (NPC) is an epithelial squamous cell carcinoma on the mucosal lining of the nasopharynx. The etiology of NPC remains elusive despite many reported studies. Most studies employ a single platform approach, neglecting the cumulative influence of both the genome and transcriptome toward NPC development. We aim to employ an integrated pathway approach to identify dysregulated pathways linked to NPC. Our approach combines imputation NPC GWAS data from a Malaysian cohort as well as published expression data GSE12452 from both NPC and non\u2010NPC nasopharynx tissues. Pathway association for GWAS data was performed using MAGENTA while for expression data, GSA\u2010SNP was used with gene <jats:italic>p<\/jats:italic> values derived from differential expression values from GEO2R. Our study identified NPC association in the gene ontology (GO) axonemal dynein complex pathway (<jats:italic>p<\/jats:italic><jats:sub>GWAS-GSEA<\/jats:sub>\u2009=\u20091.98 \u00d7 10<jats:sup>\u22122<\/jats:sup>; <jats:italic>p<\/jats:italic><jats:sub>Expr\u2010GSEA<\/jats:sub>\u2009=\u20091.27 \u00d7 10<jats:sup>\u221224<\/jats:sup>; <jats:italic>p<\/jats:italic><jats:sub>Bonf\u2010Combined<\/jats:sub>\u2009=\u20094.15 \u00d7 10<jats:sup>\u221221<\/jats:sup>). This association was replicated in a separate cohort using gene expression data from NPC and non\u2010NPC nasopharynx tissues (<jats:italic>p<\/jats:italic><jats:sub>AmpliSeq\u2010GSEA<\/jats:sub>\u2009=\u20096.56 \u00d7 10<jats:sup>\u22124<\/jats:sup>). Loss of function in the axonemal dynein complex causes impaired cilia function, leading to poor mucociliary clearance and subsequently upper or lower respiratory tract infection, the former of which includes the nasopharynx. Our approach illustrates the potential use of integrated pathway analysis in detecting gene sets involved in the development of NPC in the Malaysian cohort.<\/jats:p>","DOI":"10.1002\/ijc.30207","type":"journal-article","created":{"date-parts":[[2016,5,28]],"date-time":"2016-05-28T13:52:46Z","timestamp":1464443566000},"page":"1731-1739","source":"Crossref","is-referenced-by-count":9,"title":["Integrated pathway analysis of nasopharyngeal carcinoma implicates the axonemal dynein complex in the Malaysian cohort"],"prefix":"10.1002","volume":"139","author":[{"given":"Yoon\u2010Ming","family":"Chin","sequence":"first","affiliation":[{"name":"Institute of Biological Sciences, Faculty of Science, University of Malaya Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu Ping","family":"Tan","sequence":"additional","affiliation":[{"name":"Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research Kuala Lumpur 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MARA (UiTM)  Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geok Wee","family":"Tan","sequence":"additional","affiliation":[{"name":"Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan Soo\u2010Beng","family":"Khoo","sequence":"additional","affiliation":[{"name":"Molecular Pathology Unit, Cancer Research Centre, Institute for Medical Research Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gopala","family":"Krishnan","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Faculty of Medicine, University of Malaya Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kin\u2010Choo","family":"Pua","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Pulau Pinang General Hospital Georgetown Pulau Pinang 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