{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T07:20:56Z","timestamp":1790234456185,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,6,24]],"date-time":"2016-06-24T00:00:00Z","timestamp":1466726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2016,10,15]]},"abstract":"<jats:p><jats:italic>TP53<\/jats:italic> abnormalities lead to resistance to purine analogues and are found in over 40% of patients with refractory chronic lymphocytic leukemia (CLL). At diagnosis, no more than 5% of patients carry the 17p deletion, most cases harbour mutations within the other <jats:italic>TP53<\/jats:italic> allele. The incidence of a <jats:italic>TP53<\/jats:italic> mutation as the only alteration is approximately 5%, but this depends on the sensitivity of the technique. Recently, having a complex karyotype has been considered a strong adverse prognostic factor. However, there are no longitudinal studies simultaneously examining the presence of the 17p deletion, <jats:italic>TP53<\/jats:italic> mutations and karyotype abnormalities. We conducted a retrospective longitudinal study of 31 relapsed\/refractory CLL patients. Two to six blood samples per patient were analyzed, with a median follow\u2010up of 8 years. In this report, we assessed the sequence of events of <jats:italic>TP53<\/jats:italic> clonal evolution and correlated the presence of <jats:italic>TP53<\/jats:italic> abnormalities to genetic instability during progression and treatment. Next\u2010generation sequencing allowed the early detection of <jats:italic>TP53<\/jats:italic> mutated clones and was able to be performed on a routine basis, demonstrating an excellent correlation between the Illumina and Ion Torrent technologies. We concluded that <jats:italic>TP53<\/jats:italic> mutations are early events and precede clonal evolution to complex karyotypes. We strongly recommend the early and iterated detection of <jats:italic>TP53<\/jats:italic> mutations in progressive cases.<\/jats:p>","DOI":"10.1002\/ijc.30222","type":"journal-article","created":{"date-parts":[[2016,6,8]],"date-time":"2016-06-08T00:20:12Z","timestamp":1465345212000},"page":"1759-1763","source":"Crossref","is-referenced-by-count":23,"title":["TP53 mutations are early events in chronic lymphocytic leukemia disease progression and precede evolution to complex karyotypes"],"prefix":"10.1002","volume":"139","author":[{"given":"Gregory","family":"Lazarian","sequence":"first","affiliation":[{"name":"U978 Institut National De La Sant\u00e9 Et De La Recherche M\u00e9dicale Bobigny France"},{"name":"Labex Inflamex Universit\u00e9 Paris 13, Sorbonne Paris Cit\u00e9 Bobigny France"},{"name":"H\u00f4pital Avicenne, Assistance Publique\u2014H\u00f4pitaux De Paris, Bobigny Service D'H\u00e9matologie Biologique  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugen","family":"Tausch","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III Ulm University Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Eclache","sequence":"additional","affiliation":[{"name":"U978 Institut National De La Sant\u00e9 Et De La Recherche M\u00e9dicale Bobigny France"},{"name":"H\u00f4pital Avicenne, Assistance Publique\u2014H\u00f4pitaux De Paris, Bobigny Service D'H\u00e9matologie Biologique  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amel","family":"Sebaa","sequence":"additional","affiliation":[{"name":"H\u00f4pital Avicenne, Assistance Publique\u2014H\u00f4pitaux De Paris, Bobigny Service D'H\u00e9matologie Biologique  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Bianchi","sequence":"additional","affiliation":[{"name":"H\u00f4pital Avicenne, Assistance Publique\u2014H\u00f4pitaux De Paris, Bobigny Service D'H\u00e9matologie Biologique  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Remi","family":"Letestu","sequence":"additional","affiliation":[{"name":"U978 Institut National De La Sant\u00e9 Et De La Recherche M\u00e9dicale Bobigny France"},{"name":"Labex Inflamex Universit\u00e9 Paris 13, Sorbonne Paris Cit\u00e9 Bobigny France"},{"name":"H\u00f4pital Avicenne, Assistance Publique\u2014H\u00f4pitaux De Paris, Bobigny Service D'H\u00e9matologie Biologique  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Francois","family":"Collon","sequence":"additional","affiliation":[{"name":"H\u00f4pital Avicenne, Assistance Publique\u2014H\u00f4pitaux De Paris, Bobigny Service D'H\u00e9matologie Biologique  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie","family":"Lefebvre","sequence":"additional","affiliation":[{"name":"H\u00f4pital Avicenne, Assistance Publique\u2014H\u00f4pitaux De Paris, Bobigny Service D'H\u00e9matologie Biologique  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Gardano","sequence":"additional","affiliation":[{"name":"U978 Institut National De La Sant\u00e9 Et De La Recherche M\u00e9dicale Bobigny France"},{"name":"Labex Inflamex Universit\u00e9 Paris 13, Sorbonne Paris Cit\u00e9 Bobigny France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Varin\u2010Blank","sequence":"additional","affiliation":[{"name":"U978 Institut National De La Sant\u00e9 Et De La Recherche M\u00e9dicale Bobigny France"},{"name":"Labex Inflamex Universit\u00e9 Paris 13, Sorbonne Paris Cit\u00e9 Bobigny France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Soussi","sequence":"additional","affiliation":[{"name":"Department of Oncology\u2010Pathology Karolinska Institutet, Cancer Center Karolinska (CCK) R8:04, Stockholm SE\u2010171 76, Sweden; Sorbonne Universit\u00e9s, UPMC Univ Paris 06 Paris F\u201075005 France"},{"name":"INSERM, U1138, Centre de Recherche des Cordeliers, Paris, France and Universit\u00e9 Paris Descartes Sorbonne Paris Cit\u00e9, Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Stilgenbauer","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III Ulm University Ulm Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Cymbalista","sequence":"additional","affiliation":[{"name":"U978 Institut National De La Sant\u00e9 Et De La Recherche M\u00e9dicale Bobigny France"},{"name":"Labex Inflamex Universit\u00e9 Paris 13, Sorbonne Paris Cit\u00e9 Bobigny France"},{"name":"H\u00f4pital Avicenne, Assistance Publique\u2014H\u00f4pitaux De Paris, Bobigny Service D'H\u00e9matologie Biologique  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanny","family":"Baran\u2010Marszak","sequence":"additional","affiliation":[{"name":"U978 Institut National De La Sant\u00e9 Et De La Recherche M\u00e9dicale Bobigny France"},{"name":"Labex Inflamex Universit\u00e9 Paris 13, Sorbonne Paris Cit\u00e9 Bobigny France"},{"name":"H\u00f4pital Avicenne, Assistance Publique\u2014H\u00f4pitaux De Paris, Bobigny Service D'H\u00e9matologie Biologique  France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,24]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2008.274"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2010.32.0838"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-08-1630"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-04-154070"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-05-224071"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-11-539726"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-03-585075"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.25"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.29566"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2009.06.004"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22293"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.21993"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.22552"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature15395"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-07-659144"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2762"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2014.297"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2014.108365"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.30222","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.30222","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T13:45:20Z","timestamp":1696340720000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.30222"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,24]]},"references-count":18,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2016,10,15]]}},"alternative-id":["10.1002\/ijc.30222"],"URL":"https:\/\/doi.org\/10.1002\/ijc.30222","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,24]]}}}