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Here, we studied the suitability of Cetuximab, a therapeutic monoclonal antibody targeting the human epidermal growth factor receptor (EGFR), near\u2010infrared (NIR) fluorescently labeled as a new tool for fluorescence\u2010guided surgery. Distribution and binding of systemically injected Cetuximab Alexa Fluor 647 conjugate (Cetux\u2010Alexa\u2010647) and the co\u2010injected control human IgG Alexa Fluor 750 conjugate (hIgG\u2010Alexa\u2010750) was studied over 48 h by NIR fluorescence imaging in mice bearing human orthotopic AsPC\u20101 and MIA PaCa\u20102 PDAC tumors. Cetux\u2010Alexa\u2010647, but not the control hIgG\u2010Alexa\u2010750 fluorescence, was specifically detected <jats:italic>in vivo<\/jats:italic> in both primary pancreatic tumors with maximum fluorescence intensities at 24 h, and in metastases of AsPC\u20101 tumors as small as 1 mm. Lifetime analysis and NIR fluorescence microscopy of tumor sections confirmed the binding specificity of Cetux\u2010Alexa\u2010647 to PDAC cells. Comparable results were obtained with Cetuximab conjugated to Alexa Fluor 750 dye (Cetux\u2010Alexa\u2010750). Fluorescence\u2010guided dissection, performed 24 h after injection of Cetuximab conjugated to IRDye 800CW (Cetux\u2010800CW), enabled a real\u2010time delineation of AsPC\u20101 tumor margins, and small metastases. Odyssey scans revealed that only the vital part of the tumor, but not the necrotic part was stained with Cetux\u2010800CW. NIR fluorescently labeled Cetuximab may be a promising tool that can be applied for fluorescence\u2010guided surgery to visualize tumor margins and metastatic sites in order to allow a precise surgical resection.<\/jats:p>","DOI":"10.1002\/ijc.30277","type":"journal-article","created":{"date-parts":[[2016,7,18]],"date-time":"2016-07-18T23:06:55Z","timestamp":1468883215000},"page":"2277-2289","source":"Crossref","is-referenced-by-count":23,"title":["Preclinical evaluation of near\u2010infrared (NIR) fluorescently labeled cetuximab as a potential tool for fluorescence\u2010guided surgery"],"prefix":"10.1002","volume":"139","author":[{"given":"Mara","family":"Saccomano","sequence":"first","affiliation":[{"name":"Department of Molecular Biology of Neuronal Signals Max\u2010Planck\u2010Institute of Experimental Medicine G\u00f6ttingen 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