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PDT is cytotoxic, provokes anti\u2010vascular effects and stimulates tumor cell targeting mechanisms of the immune system and, consequently, has potential as a novel therapy for osteosarcoma patients. This study investigated the uptake and the dark\u2010 and phototoxicity and cytotoxic mechanisms of the photosensitizer (PS) 5,10,15,20\u2010tetrakis(meta\u2010hydroxyphenyl) chlorine (mTHPC, Foscan) and a liposomal mTHPC formulation (Foslip) in the human 143B and a mouse K7M2\u2010derived osteosaroma cell line (K7M2L2) <jats:italic>in vitro<\/jats:italic>. Second, the tumor\u2010 and metastasis\u2010suppressive efficacies of mTHPC formulations based PDT and associated mechanisms in intratibial, metastasizing osteosarcoma mouse models (143B\/SCID and syngeneic K7M2L2\/BALB\/c) were studied. The uptake of Foscan and Foslip <jats:italic>in vitro<\/jats:italic> was time\u2010 and dose\u2010dependent and resulted in mTHPC and light dose\u2010dependent phototoxicity associated with apoptosis. <jats:italic>In vivo,<\/jats:italic> the uptake of both i.v. administered mTHPC formulations was higher in tumor than in healthy control tissue. PDT caused significant (Foscan <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05, Foslip <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) tumor growth inhibition in both models. A significant (Foscan <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001, Foslip <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) immune system\u2010dependent suppression of lung metastasis was only observed in the K7M2L2\/BALB\/c model and was associated with a marked infiltration of T\u2010lymphocytes at the primary tumor site. In conclusion, mTHPC\u2010based PDT is effective in clinically relevant experimental osteosarcoma and suppresses lung metastasis in immunocompetent mice with beneficial effects of the liposomal mTHPC formulation Foslip.<\/jats:p>","DOI":"10.1002\/ijc.30572","type":"journal-article","created":{"date-parts":[[2016,12,10]],"date-time":"2016-12-10T23:24:37Z","timestamp":1481412277000},"page":"1680-1692","source":"Crossref","is-referenced-by-count":44,"title":["Foscan and foslip based photodynamic therapy in osteosarcoma <i>in vitro<\/i> and in intratibial mouse models"],"prefix":"10.1002","volume":"140","author":[{"given":"Daniela","family":"Meier","sequence":"first","affiliation":[{"name":"Laboratory for Orthopedic Research, Department of Orthopedics Balgrist University Hospital Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sander M.","family":"Botter","sequence":"additional","affiliation":[{"name":"Laboratory for Orthopedic Research, Department of 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