{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T21:47:22Z","timestamp":1790459242021,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,7,12]],"date-time":"2017-07-12T00:00:00Z","timestamp":1499817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"SiRIC Curie","award":["INCa-DGOS-4654"],"award-info":[{"award-number":["INCa-DGOS-4654"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2017,10,15]]},"abstract":"<jats:p>Squamous cell carcinoma of the anal canal (SCCA) is a rare HPV\u2010associated cancer with limited sensitivity to standard chemotherapy. In a phase 2 study, nivolumab, an anti PD\u20101 immune checkpoint inhibitor, demonstrated significant efficacy as single\u2010agent therapy in metastatic SCCA patients. Nevertheless, imaging assessment by standard RECIST criteria of the efficacy of immune therapy can be difficult in some patients due to tumor immune cell infiltration, and biomarkers of treatment efficacy are needed. We have previously developed a quantitative droplet digital PCR (ddPCR) technique to detect HPV circulating tumor DNA (HPV ctDNA), with excellent sensitivity and specificity. Here, we report, for the first time, the kinetics of HPV ctDNA during therapy in a patient with metastatic SCCA, who obtained sustained partial response to single\u2010agent nivolumab. We observed an early and very significant decrease of HPV ctDNA during therapy from the baseline level of 3713 copies\/ml plasma to 564 copies\/ml plasma at 4 weeks, and 156 copies\/ml at 6 weeks, followed by a plateau. This observation provides proof\u2010of\u2010concept that HPV ctDNA can be used as a noninvasive early dynamic biomarker to monitor the efficacy of new immunotherapy agents.<\/jats:p>","DOI":"10.1002\/ijc.30863","type":"journal-article","created":{"date-parts":[[2017,7,3]],"date-time":"2017-07-03T08:40:47Z","timestamp":1499071247000},"page":"1667-1670","source":"Crossref","is-referenced-by-count":34,"title":["HPV circulating tumor DNA to monitor the efficacy of anti\u2010PD\u20101 therapy in metastatic squamous cell carcinoma of the anal canal: A case report"],"prefix":"10.1002","volume":"141","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5515-9180","authenticated-orcid":false,"given":"Luc","family":"Cabel","sequence":"first","affiliation":[{"name":"Department of Medical Oncology Institut Curie, PSL Research University Paris and Saint Cloud France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois\u2010Cl\u00e9ment","family":"Bidard","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Institut Curie, PSL Research University Paris and Saint Cloud France"},{"name":"Versailles Saint\u2010Quentin\u2010en\u2010Yvelines &amp; Paris\u2010Saclay University Saint Cloud France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Servois","sequence":"additional","affiliation":[{"name":"Department of Radiology Institut Curie, PSL Research University Paris and Saint Cloud France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wulfran","family":"Cacheux","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Institut Curie, PSL Research University Paris and Saint Cloud France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascale","family":"Mariani","sequence":"additional","affiliation":[{"name":"Department of Surgery Institut Curie, PSL Research University Paris and Saint Cloud France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela","family":"Romano","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Institut Curie, PSL Research University Paris and Saint Cloud France"},{"name":"Institut Curie, Center of Cancer Immunotherapy, INSERM U932  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Minsat","sequence":"additional","affiliation":[{"name":"Department of Radiotherapy Institut Curie, PSL Research University Paris and Saint Cloud France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Bieche","sequence":"additional","affiliation":[{"name":"Department of Genetics Institut Curie, PSL Research University Paris and Saint Cloud France"},{"name":"Paris Descartes University Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fereshteh","family":"Farkhondeh","sequence":"additional","affiliation":[{"name":"Department of Pathology Institut Curie, PSL Research University Paris and Saint Cloud France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Jeannot","sequence":"additional","affiliation":[{"name":"Department of Genetics Institut Curie, PSL Research University Paris and Saint Cloud France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Buecher","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Institut Curie, PSL Research University Paris and Saint Cloud France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21332"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25671"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11912-016-0503-3"},{"key":"e_1_2_5_5_1","unstructured":"NCCN. Anal carcinoma. Clinical Practice Guidelines in Oncology (NCCN Guidelines\u00ae). Version I.2017. 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