{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T01:49:17Z","timestamp":1787622557575,"version":"build-2736575974"},"reference-count":52,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,10,10]],"date-time":"2017-10-10T00:00:00Z","timestamp":1507593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010438","name":"Francis Crick Institute","doi-asserted-by":"publisher","award":["FC001999"],"award-info":[{"award-number":["FC001999"]}],"id":[{"id":"10.13039\/100010438","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["FC001999"],"award-info":[{"award-number":["FC001999"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["FC001999"],"award-info":[{"award-number":["FC001999"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:p>A variety of analytical approaches have indicated that melanoma cell line UCLA\u2010SO\u2010M14 (M14) and breast carcinoma cell line MDA\u2010MB\u2010435 originate from a common donor. This indicates that at some point in the past, one of these cell lines became misidentified, meaning that it ceased to correspond to the reported donor and instead became falsely identified (through cross\u2010contamination or other means) as a cell line from a different donor. Initial studies concluded that MDA\u2010MB\u2010435 was the misidentified cell line and M14 was the authentic cell line, although contradictory evidence has been published, resulting in further confusion. To address this question, we obtained early samples of the melanoma cell line (M14), a lymphoblastoid cell line from the same donor (ML14), and donor serum preserved at the originator's institution. M14 samples were cryopreserved in December 1975, before MDA\u2010MB\u2010435 cells were established in culture. Through a series of molecular characterizations, including short tandem repeat (STR) profiling and cytogenetic analysis, we demonstrated that later samples of M14 and MDA\u2010MB\u2010435 correspond to samples of M14 frozen in 1975, to the lymphoblastoid cell line ML14, and to the melanoma donor's STR profile, sex and blood type. This work demonstrates conclusively that M14 is the authentic cell line and MDA\u2010MB\u2010435 is misidentified. With clear provenance information and authentication testing of early samples, it is possible to resolve debates regarding the origins of problematic cell lines that are widely used in cancer research.<\/jats:p>","DOI":"10.1002\/ijc.31067","type":"journal-article","created":{"date-parts":[[2017,9,22]],"date-time":"2017-09-22T08:37:20Z","timestamp":1506069440000},"page":"561-572","source":"Crossref","is-referenced-by-count":48,"title":["Authentication of M14 melanoma cell line proves misidentification of MDA\u2010MB\u2010435 breast cancer cell line"],"prefix":"10.1002","volume":"142","author":[{"given":"Christopher","family":"Korch","sequence":"first","affiliation":[{"name":"International Cell Line Authentication Committee (ICLAC)"},{"name":"Division of Medical Oncology University of Colorado Anschutz Medical Campus Aurora CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin M.","family":"Hall","sequence":"additional","affiliation":[{"name":"International Cell Line Authentication Committee (ICLAC)"},{"name":"Genetica Cell Line Testing \u2013 a LabCorp brand Burlington NC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilhelm G.","family":"Dirks","sequence":"additional","affiliation":[{"name":"International Cell Line Authentication Committee (ICLAC)"},{"name":"Leibniz\u2010Institute DSMZ \u2013 Deutsche Sammlung von Mikroorganismen und Zellkulturen Braunschweig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret","family":"Ewing","sequence":"additional","affiliation":[{"name":"Promega Corporation Madison WI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Faries","sequence":"additional","affiliation":[{"name":"John Wayne Cancer Institute Santa Monica CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marileila","family":"Varella\u2010Garcia","sequence":"additional","affiliation":[{"name":"Division of Medical Oncology University of Colorado Anschutz Medical Campus Aurora CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Robinson","sequence":"additional","affiliation":[{"name":"Division of Medical Oncology University of Colorado Anschutz Medical Campus Aurora CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas","family":"Storts","sequence":"additional","affiliation":[{"name":"International Cell Line Authentication Committee (ICLAC)"},{"name":"Promega Corporation Madison WI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline A.","family":"Turner","sequence":"additional","affiliation":[{"name":"Division of Medical Oncology University of Colorado Anschutz Medical Campus Aurora CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Medical Oncology University of Colorado Anschutz Medical Campus Aurora CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward C.","family":"Burnett","sequence":"additional","affiliation":[{"name":"International Cell Line Authentication Committee (ICLAC)"},{"name":"Culture Collections Public Health England Porton Down United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyn","family":"Healy","sequence":"additional","affiliation":[{"name":"International Cell Line Authentication Committee (ICLAC)"},{"name":"Biological Research Facility The Francis Crick Institute London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas","family":"Kniss","sequence":"additional","affiliation":[{"name":"International Cell Line Authentication Committee (ICLAC)"},{"name":"Departments of Obstetrics and Gynecology and Biomedical Engineering The Ohio State University Columbus OH"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard M.","family":"Neve","sequence":"additional","affiliation":[{"name":"International Cell Line Authentication Committee (ICLAC)"},{"name":"Gilead Sciences Inc Foster City CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond W.","family":"Nims","sequence":"additional","affiliation":[{"name":"International Cell Line Authentication Committee (ICLAC)"},{"name":"RMC Pharmaceutical Solutions, Inc. 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