{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T18:07:38Z","timestamp":1761156458765,"version":"3.37.3"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,3,30]],"date-time":"2018-03-30T00:00:00Z","timestamp":1522368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32\u20105T32AI007001","R01CA142983","R01CA142983\u201002S1"],"award-info":[{"award-number":["T32\u20105T32AI007001","R01CA142983","R01CA142983\u201002S1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:p>The role of host epigenetic mechanisms in the natural history of low\u2010grade cervical intraepithelial neoplasia (CIN1) is not well characterized. We explored differential methylation of imprinted gene regulatory regions as predictors of the risk of CIN1 regression. A total of 164 patients with CIN1 were recruited from 10 Duke University clinics for the CIN Cohort Study. Participants had colposcopies at enrollment and up to five follow\u2010up visits over 3 years. DNA was extracted from exfoliated cervical cells for methylation quantitation at CpG (cytosine\u2010phosphate\u2010guanine) sites and human papillomavirus (HPV) genotyping. Hazard ratios (HR) and 95% confidence intervals (CI) were estimated using Cox regression to quantify the effect of methylation on CIN1 regression over two consecutive visits, compared to non\u2010regression (persistent CIN1; progression to CIN2+; or CIN1 regression at a single time\u2010point), adjusting for age, race, high\u2010risk HPV (hrHPV), parity, oral contraceptive and smoking status. Median participant age was 26.6 years (range: 21.0\u201364.4 years), 39% were African\u2010American, and 11% were current smokers. Most participants were hrHPV\u2010positive at enrollment (80.5%). Over one\u2010third of cases regressed (<jats:italic>n<\/jats:italic>\u2009=\u200953, 35.1%). Median time\u2010to\u2010regression was 12.6 months (range: 4.5\u201324.0 months). Probability of CIN1 regression was negatively correlated with methylation at <jats:italic>IGF2AS<\/jats:italic> CpG 5 (HR\u2009=\u20090.41; 95% CI\u2009=\u20090.23\u20130.77) and <jats:italic>PEG10<\/jats:italic> DMR (HR\u2009=\u20090.80; 95% CI\u2009=\u20090.65\u20130.98). Altered methylation of imprinted <jats:italic>IGF2AS<\/jats:italic> and <jats:italic>PEG10<\/jats:italic> DMRs may play a role in the natural history of CIN1. If confirmed in larger studies, further research on imprinted gene DMR methylation is warranted to determine its efficacy as a biomarker for cervical cancer screening.<\/jats:p>","DOI":"10.1002\/ijc.31350","type":"journal-article","created":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:56:43Z","timestamp":1519865803000},"page":"552-560","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["DNA methylation of imprinted gene control regions in the regression of low\u2010grade cervical lesions"],"prefix":"10.1002","volume":"143","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9854-8888","authenticated-orcid":false,"given":"Ayodele","family":"Gomih","sequence":"first","affiliation":[{"name":"Department of Epidemiology University of North Carolina at Chapel Hill  NC 27599"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer S.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Epidemiology University of North Carolina at Chapel Hill  NC 27599"},{"name":"Lineberger Comprehensive Cancer Center Chapel Hill NC USA 27599"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kari E.","family":"North","sequence":"additional","affiliation":[{"name":"Department of Epidemiology University of North Carolina at Chapel Hill  NC 27599"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael G.","family":"Hudgens","sequence":"additional","affiliation":[{"name":"Department of Biostatistics University of North Carolina at Chapel Hill  NC 27599"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendy R.","family":"Brewster","sequence":"additional","affiliation":[{"name":"Lineberger Comprehensive Cancer Center Chapel Hill NC USA 27599"},{"name":"Department of Obstetrics and Gynecology University of North Carolina at Chapel Hill  NC 27599"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiqing","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Duke University School of Medicine Durham NC 27710"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Skaar","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Center for Human Health and the Environment, North Carolina State University Raleigh NC 27695"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fidel","family":"Valea","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Virginia Tech Carilion School of Medicine Roanoke VA 24101"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rex C.","family":"Bentley","sequence":"additional","affiliation":[{"name":"Department of Pathology Duke University School of Medicine Durham NC 27710"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana C.","family":"Vidal","sequence":"additional","affiliation":[{"name":"Department of Surgery Cedars\u2010Sinai Medical Center Los Angeles CA 90048"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel L.","family":"Maguire","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Center for Human Health and the Environment, North Carolina State University Raleigh NC 27695"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Randy L.","family":"Jirtle","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Center for Human Health and the Environment, North Carolina State University Raleigh NC 27695"},{"name":"Department of Oncology, McArdle Laboratory for Cancer Research University of Wisconsin\u2010Madison Madison WI 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan K.","family":"Murphy","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Duke University School of Medicine Durham NC 27710"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cathrine","family":"Hoyo","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Center for Human Health and the Environment, North Carolina State University Raleigh NC 27695"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,30]]},"reference":[{"volume-title":"SEER Cancer Stat Facts: Cervix Uteri Cancer","year":"2016","author":"United States National Cancer Institute","key":"e_1_2_5_2_1"},{"key":"e_1_2_5_3_1","unstructured":"U.S. Cancer Statistics Working Group. 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