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Primary cell\u2010based <jats:italic>in vitro<\/jats:italic> models of NSCLC are therefore desirable for novel therapy development and personalized cancer medicine. Methods have been described to generate rapidly proliferating epithelial cell cultures from multiple human epithelia using 3T3\u2010J2 feeder cell culture in the presence of Y\u201027632, a RHO\u2010associated protein kinase (ROCK) inhibitor, in what are known as \u201cconditional reprograming conditions\u201d (CRC) or 3T3\u2009+\u2009Y. In some cancer studies, variations of this methodology have allowed primary tumor cell expansion across a number of cancer types but other studies have demonstrated the preferential expansion of normal epithelial cells from tumors in such conditions. Here, we report our experience regarding the derivation of primary NSCLC cell cultures from 12 lung adenocarcinoma patients enrolled in the Tracking Cancer Evolution through Therapy (TRACERx) clinical study and discuss these in the context of improving the success rate for <jats:italic>in vitro<\/jats:italic> cultivation of cells from NSCLC tumors.<\/jats:p>","DOI":"10.1002\/ijc.31383","type":"journal-article","created":{"date-parts":[[2018,3,23]],"date-time":"2018-03-23T04:59:28Z","timestamp":1521781168000},"page":"160-166","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Expansion of airway basal epithelial cells from primary human non\u2010small cell lung cancer tumors"],"prefix":"10.1002","volume":"143","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2170-8791","authenticated-orcid":false,"given":"Robert E.","family":"Hynds","sequence":"first","affiliation":[{"name":"UCL Cancer Institute, CRUK Lung Cancer Centre of Excellence University College London London United Kingdom"},{"name":"Translational Cancer Therapeutics Laboratory The Francis Crick Institute London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Assma","family":"Ben Aissa","sequence":"additional","affiliation":[{"name":"Cancer Immunology Unit UCL Cancer Institute, University College London London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate H.C.","family":"Gowers","sequence":"additional","affiliation":[{"name":"Lungs for Living Research Centre, UCL Respiratory University College London London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas B.K.","family":"Watkins","sequence":"additional","affiliation":[{"name":"Translational Cancer Therapeutics Laboratory The Francis Crick Institute London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leticia","family":"Bosshard\u2010Carter","sequence":"additional","affiliation":[{"name":"UCL Cancer Institute, CRUK Lung Cancer Centre of Excellence University College London London United Kingdom"},{"name":"Lungs for Living Research Centre, UCL Respiratory University College London London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew J.","family":"Rowan","sequence":"additional","affiliation":[{"name":"Translational Cancer Therapeutics Laboratory The Francis Crick Institute London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selvaraju","family":"Veeriah","sequence":"additional","affiliation":[{"name":"UCL Cancer Institute, CRUK Lung Cancer Centre of Excellence University College London London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gareth A.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Translational Cancer Therapeutics Laboratory The Francis Crick Institute London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio A.","family":"Quezada","sequence":"additional","affiliation":[{"name":"Cancer Immunology Unit UCL Cancer Institute, University College London London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Swanton","sequence":"additional","affiliation":[{"name":"UCL Cancer Institute, CRUK Lung Cancer Centre of Excellence University College London London United Kingdom"},{"name":"Translational Cancer Therapeutics Laboratory The Francis Crick Institute London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"on behalf of the TRACERx Consortium","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sam M.","family":"Janes","sequence":"additional","affiliation":[{"name":"Lungs for Living Research Centre, UCL Respiratory University College London London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,4,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI42297"},{"key":"e_1_2_6_3_1","first-page":"1363","article-title":"Efficient procurement of epithelial stem cells from human tissue specimens using a Rho\u2010associated protein kinase inhibitor Y\u201027632. 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