{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T23:08:06Z","timestamp":1791932886790,"version":"4.3.4"},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,4,27]],"date-time":"2018-04-27T00:00:00Z","timestamp":1524787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004622","name":"Dutch Cancer Society","doi-asserted-by":"publisher","award":["KWF VU 2014\u20107238"],"award-info":[{"award-number":["KWF VU 2014\u20107238"]}],"id":[{"id":"10.13039\/501100004622","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["603019"],"award-info":[{"award-number":["603019"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2018,9,15]]},"abstract":"<jats:p>\n                    DNA methylation analysis of cervical scrapes using\n                    <jats:italic>FAM19A4<\/jats:italic>\n                    and\n                    <jats:italic>mir124\u20102<\/jats:italic>\n                    genes has shown a good clinical performance in detecting cervical cancer and advanced CIN lesions in need of treatment in HPV\u2010positive women. To date, longitudinal data on the cancer risk of methylation test\u2010negative women are lacking. In our study, we assessed the longitudinal outcome of\n                    <jats:italic>FAM19A4\/mir124\u20102<\/jats:italic>\n                    methylation analysis in an HPV\u2010positive screening cohort with 14 years of follow\u2010up. Archived HPV\u2010positive cervical scrapes of 1,040 women (age 29\u201361 years), who were enrolled in the POBASCAM screening trial (ISRCTN20781131) were tested for\n                    <jats:italic>FAM19A4<\/jats:italic>\n                    \/\n                    <jats:italic>mir124\u20102<\/jats:italic>\n                    methylation. By linkage with the nationwide network and registry of histo\u2010 and cytopathology in the Netherlands (PALGA), 35 cervical cancers were identified during 14 years of follow\u2010up comprising three screens (baseline, and after 5 and 10 years). The baseline scrape of 36.1% (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a0375) women tested positive for\n                    <jats:italic>FAM19A4\/mir124\u20102<\/jats:italic>\n                    methylation, including 24 women with cervical cancer in follow\u2010up, and 30.6% (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a0318) had abnormal cytology (threshold borderline dyskaryosis or ASCUS), including 14 women with cervical cancer in follow\u2010up. Within screening round capability of\n                    <jats:italic>FAM19A4\/mir124\u20102<\/jats:italic>\n                    methylation to detect cervical cancer was 100% (11\/11, 95% CI: 71.5\u2013100). Kaplan\u2013Meier estimate of 14\u2010year cumulative cervical cancer incidence was 1.7% (95% CI: 0.66\u20133.0) among baseline methylation\u2010negative and 2.4% (95% CI: 1.4\u20133.6) among baseline cytology\u2010negative women (risk difference: 0.71% [95% CI: 0.16\u20131.4]). In conclusion, a negative\n                    <jats:italic>FAM19A4\/mir124\u20102<\/jats:italic>\n                    methylation test provides a low cervical cancer risk in HPV\u2010positive women of 30 years and older.\n                    <jats:italic>FAM19A4\/mir124\u20102<\/jats:italic>\n                    methylation testing merits consideration as an objective triage test in HPV\u2010based cervical screening programs.\n                  <\/jats:p>","DOI":"10.1002\/ijc.31539","type":"journal-article","created":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T00:28:34Z","timestamp":1523924914000},"page":"1541-1548","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":104,"title":["Cervical cancer risk in HPV\u2010positive women after a negative\n                    <i>FAM19A4\/mir124\u20102<\/i>\n                    methylation test: A post hoc analysis in the POBASCAM trial with 14 year follow\u2010up"],"prefix":"10.1002","volume":"143","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1737-2295","authenticated-orcid":false,"given":"Lise M.A.","family":"De Strooper","sequence":"first","affiliation":[{"name":"Department of Pathology Cancer Center Amsterdam, VU University Medical Center Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Berkhof","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Biostatistics VU University Medical Center Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renske D.M.","family":"Steenbergen","sequence":"additional","affiliation":[{"name":"Department of Pathology Cancer Center Amsterdam, VU University Medical Center Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit I.","family":"Lissenberg\u2010Witte","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Biostatistics VU University Medical Center Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.F.","family":"Snijders","sequence":"additional","affiliation":[{"name":"Department of Pathology Cancer Center Amsterdam, VU University Medical Center Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris J.L.M.","family":"Meijer","sequence":"additional","affiliation":[{"name":"Department of Pathology Cancer Center Amsterdam, VU University Medical Center Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dani\u00eblle A.M.","family":"Heideman","sequence":"additional","affiliation":[{"name":"Department of Pathology Cancer Center Amsterdam, VU University Medical Center Amsterdam The 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