{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T10:08:43Z","timestamp":1769076523224,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,11,12]],"date-time":"2018-11-12T00:00:00Z","timestamp":1541980800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP26870685"],"award-info":[{"award-number":["JP26870685"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:p>Molecular irreversibleness with <jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H. pylori<\/jats:italic>) infection might have a role in gastric tumorigenesis after <jats:italic>H. pylori<\/jats:italic> eradication. We performed comprehensive DNA methylation profiling of gastric mucosa after <jats:italic>H. pylori<\/jats:italic> eradication with or without gastric cancer. Using four different groups of biopsies obtained from gastric body without history of <jats:italic>H. pylori<\/jats:italic> infection (Hp\u2010), gastric body without cancer after <jats:italic>H. pylori<\/jats:italic> eradication (cancer\u2010free body), gastric body with early gastric cancer diagnosed after <jats:italic>H. pylori<\/jats:italic> eradication (EGC body) and their paired samples from adjacent mucosa of cancer (EGC ADJ), methylation status of five candidate genes (<jats:italic>MYOD1<\/jats:italic>, <jats:italic>SLC16A12<\/jats:italic>, <jats:italic>IGF2<\/jats:italic>, <jats:italic>RORA<\/jats:italic> and <jats:italic>PRDM5<\/jats:italic>) was examined by the bisulfite pyrosequencing. An Infinium Methylation EPIC BeadChip array was also used to characterize the methylation status of greater than 850,000 CpG sites. The EGC ADJ group showed highest methylation levels of five candidate genes among the four groups of biopsies. In the gastric body (cancer\u2010free body + EGC body), methylation levels were significantly decreased in patients with longer period after eradication, while such association was not observed in EGC ADJ group. Hyper methylated samples were associated with shorter telomere, an indicator for rapid cell turnover, and higher DNMT1 protein expression, an enzyme related to methyl transfer reaction. The genome\u2010wide methylation analysis demonstrated strikingly higher methylation levels especially at CpG islands in the EGC ADJ group. Exclusively hypermethylated promoter CpG islands in the same group frequently coded zinc finger proteins. Our data show that DNA methylation accumulation is associated with molecular irreversibleness and gastric carcinogenesis after <jats:italic>H. pylori<\/jats:italic> eradication.<\/jats:p>","DOI":"10.1002\/ijc.31667","type":"journal-article","created":{"date-parts":[[2018,7,6]],"date-time":"2018-07-06T08:40:02Z","timestamp":1530866402000},"page":"80-88","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["DNA methylation accumulation in gastric mucosa adjacent to cancer after <i>Helicobacter pylori<\/i> eradication"],"prefix":"10.1002","volume":"144","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1901-5056","authenticated-orcid":false,"given":"Sayumi","family":"Tahara","sequence":"first","affiliation":[{"name":"Department of Diagnostic Pathology I School of Medicine, Fujita Health University  Toyoake 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