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Human tumor tropic mesenchymal stem cells (MSCs) isolated from various tissues and MSCs engineered to express the yeast cytosine deaminase::uracil phosphoribosyl transferase suicide fusion gene (yCD::UPRT\u2010MSCs) released exosomes in conditional medium (CM). Exosomes from all tissue specific yCD::UPRT\u2010MSCs contained mRNA of the suicide gene in the exosome's cargo. When the CM was applied to tumor cells, the exosomes were internalized by recipient tumor cells and in the presence of the prodrug 5\u2010fluorocytosine (5\u2010FC) effectively triggered dose\u2010dependent tumor cell death by endocytosed exosomes via an intracellular conversion of the prodrug 5\u2010FC to 5\u2010fluorouracil. Exosomes were found to be responsible for the tumor inhibitory activity. The presence of microRNAs in exosomes produced from naive MSCs and from suicide gene transduced MSCs did not differ significantly. MicroRNAs from yCD::UPRT\u2010MSCs were not associated with therapeutic effect. 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