{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T13:16:28Z","timestamp":1784034988914,"version":"3.55.0"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,10,4]],"date-time":"2018-10-04T00:00:00Z","timestamp":1538611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100001424","name":"ALF","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001424","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002794","name":"Cancerfonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:p>Randomized clinical trials using human papillomavirus (HPV) DNA testing have found increased protection against cervical cancer and HPV\u2010based screening is globally recommended for women \u226530 years of age. HPV\u2010mRNA is a promising alternative target for cervical screening tests, but assessing equivalence requires longitudinal evaluation over at least the length of a screening interval. Our aim is to analyze the longitudinal sensitivity of HPV\u2010mRNA and HPV\u2010DNA in cervical samples taken up to 7 years before severe cervical intraepithelial neoplasia or worse (CIN3+). From a population\u2010based cohort of 95,023 women in Sweden, cervical samples were frozen at \u221280\u00b0C between May 2007 and January 2012. Registry linkages identified that 1,204 of these women had CIN3+ 4 months to 7 years after enrolment. Baseline samples were analyzed for HPV\u2010mRNA (Aptima, Hologic) and for HPV\u2010DNA (Cobas 4800, Roche) and results from both tests obtained for 1,172 women. For both women &lt;30 and \u2265 30 years, HPV\u2010mRNA had similar sensitivity for CIN3+ as HPV\u2010DNA (<jats:italic>p =<\/jats:italic> 0.0217 and <jats:italic>p =<\/jats:italic> 0.0123 in noninferiority testing for at least 90% relative sensitivity, respectively). Among women \u226530 years, the longitudinal sensitivities for CIN3+ occurring 5\u20137 years later were comparable [76.3% (95% CI: 65.8%\u201384.3%) and 82.5% (95% CI: 72.6%\u201389.4%)] as were the longitudinal negative predictive values for absence of CIN3+ [99.97% (95% CI: 99.95\u201399.98) and 99.98% (95% CI: 99.96\u201399.99)], for the HPV\u2010mRNA and HPV\u2010DNA test. In conclusion, HPV\u2010mRNA testing has similar longitudinal sensitivity as HPV\u2010DNA, implying that HPV\u2010mRNA testing can safely be used for cervical screening.<\/jats:p>","DOI":"10.1002\/ijc.31819","type":"journal-article","created":{"date-parts":[[2018,8,20]],"date-time":"2018-08-20T08:11:37Z","timestamp":1534752697000},"page":"1073-1081","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["HPV\u2010mRNA and HPV\u2010DNA detection in samples taken up to seven years before severe dysplasia of cervix uteri"],"prefix":"10.1002","volume":"144","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6293-7286","authenticated-orcid":false,"given":"Ola","family":"Forslund","sequence":"first","affiliation":[{"name":"Laboratory Medicine, Medical Microbiology Lund University  Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Miriam Elfstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Karolinska Institutet  Sweden"},{"name":"Unit for Screening Regional Cancer Center of Stockholm\u2010Gotland  Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Lamin","sequence":"additional","affiliation":[{"name":"Karolinska University Laboratory Karolinska University Hospital  Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joakim","family":"Dillner","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Karolinska Institutet  Sweden"},{"name":"Karolinska University Laboratory Karolinska University Hospital  Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijgo.12187"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21955"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)62218-7"},{"key":"e_1_2_6_5_1","volume-title":"European guidelines for quality assurance in cervical cancer screening","author":"Anttila A","year":"2015"},{"key":"e_1_2_6_6_1","unstructured":"WHO guidelines for screening and treatment of precancerous lesions for cervical cancer prevention.2013."},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24010"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2015.04.015"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2006.12.005"},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1001\/jama.1989.03430070079034","volume":"262","author":"The 1988 Bethesda System for reporting cervical\/vaginal cytological diagnoses. 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