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Molecular markers directly applicable on self\u2010samples are needed to stratify HPV\u2010positive women at risk of cervical cancer (so\u2010called triage) and to avoid over\u2010referral and overtreatment. Deregulated microRNAs (miRNAs) have been implicated in the development of cervical cancer, and represent potential triage markers. However, it is unknown whether deregulated miRNA expression is reflected in self\u2010samples. Our study is the first to establish genome\u2010wide miRNA profiles in HPV\u2010positive self\u2010samples to identify miRNAs that can predict the presence of CIN3 and cervical cancer in self\u2010samples. Small RNA sequencing (sRNA\u2010Seq) was conducted to determine genome\u2010wide miRNA expression profiles in 74 HPV\u2010positive self\u2010samples of women with and without cervical precancer (CIN3). The optimal miRNA marker panel for CIN3 detection was determined by GRridge, a penalized method on logistic regression. Six miRNAs were validated by qPCR in 191 independent HPV\u2010positive self\u2010samples. Classification of sRNA\u2010Seq data yielded a 9\u2010miRNA marker panel with a combined area under the curve (AUC) of 0.89 for CIN3 detection. Validation by qPCR resulted in a combined AUC of 0.78 for CIN3+ detection. Our study shows that deregulated miRNA expression associated with CIN3 and cervical cancer development can be detected by sRNA\u2010Seq in HPV\u2010positive self\u2010samples. Validation by qPCR indicates that miRNA expression analysis offers a promising novel molecular triage strategy for CIN3 and cervical cancer detection applicable to self\u2010samples.<\/jats:p>","DOI":"10.1002\/ijc.31855","type":"journal-article","created":{"date-parts":[[2018,9,7]],"date-time":"2018-09-07T15:19:11Z","timestamp":1536333551000},"page":"372-379","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Genome\u2010wide microRNA analysis of HPV\u2010positive self\u2010samples yields novel triage markers for early detection of cervical cancer"],"prefix":"10.1002","volume":"144","author":[{"given":"Barbara C.","family":"Snoek","sequence":"first","affiliation":[{"name":"Amsterdam UMC Vrije Universiteit Amsterdam, Pathology, Cancer Center Amsterdam Amsterdam Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wina","family":"Verlaat","sequence":"additional","affiliation":[{"name":"Amsterdam 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