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We conducted whole genome expression microarrays on 64 primary HCC tumors with clinically defined recurrence status and cross\u2010referenced with RNA\u2010seq data from 18 HCC tumors in the Cancer Genome Atlas project. We identified a 77\u2010gene signature, which is significantly associated with early recurrent (ER) HCC tumors. This ER\u2010associated signature shows significant enrichment in genes involved in cell cycle pathway. We performed receiver operating characteristic (ROC) analysis to evaluate the prognostic biomarker potential of these 77 genes and Pearson correlation analysis to identify 11 close clusters. The one gene with the best area under the ROC curve in each of the 11 clusters was selected for validation using reverse\u2010transcription quantitative PCR in an independent cohort of 24 HCC tumors. NUF2 was identified to be the minimal biomarker sufficient to discriminate ER tumors from LR tumors. NUF2 in combination with liver cirrhosis could significantly improve the detection of ER tumors with an AUROC of 0.82 and 0.85 in the test and validation cohort, respectively. In conclusion, NUF2 in combination with liver cirrhosis is a promising prognostic biomarker for early HCC recurrence.<\/jats:p>","DOI":"10.1002\/ijc.32134","type":"journal-article","created":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T16:53:35Z","timestamp":1547744015000},"page":"662-670","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["NUF2 is a valuable prognostic biomarker to predict early recurrence of hepatocellular carcinoma after surgical resection"],"prefix":"10.1002","volume":"145","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6894-8533","authenticated-orcid":false,"given":"Yu","family":"Wang","sequence":"first","affiliation":[{"name":"Division of Cellular and Molecular Research National Cancer Centre Singapore  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng Yang","family":"Tan","sequence":"additional","affiliation":[{"name":"Division of Cellular and Molecular 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