{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T10:16:37Z","timestamp":1790072197781,"version":"4.0.1"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,3,9]],"date-time":"2019-03-09T00:00:00Z","timestamp":1552089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["396168587"],"award-info":[{"award-number":["396168587"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["EI 866\/7\u20101"],"award-info":[{"award-number":["EI 866\/7\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["CL 427\/4\u20101 and BR 5712\/1\u20101"],"award-info":[{"award-number":["CL 427\/4\u20101 and BR 5712\/1\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2019,8,15]]},"abstract":"<jats:p>\n                    Soft tissue sarcomas (STS) are rare tumors of mesenchymal origin. About 50% of patients with STS experience relapse and more than 30% will die within 10 years after diagnosis. In this study we investigated circulating free DNA (cfDNA) and tumor\u2010specific genetic alterations therein (circulating tumor DNA, ctDNA) as diagnostic biomarkers. Plasma concentrations and fragmentation of cfDNA was analyzed with quantitative PCR. Patients with STS (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200964) had significantly higher plasma concentrations and increased fragmentation of cfDNA when compared to patients in complete remission (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200919) and healthy controls (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200941) (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.01 and\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001). Due to overlapping values between patients with STS and controls, the sensitivity and specificity of these assays is limited. Sensitive assays to detect genomic alterations in cfDNA of synovial sarcomas (t(X;18)), myxoid liposarcomas (t(12;16) and TERT C228T promoter mutation) and well\u2010differentiated\/de\u2010differentiated liposarcomas (MDM2 amplifications) were established. ctDNA was quantified in nine liposarcoma patients during the course of their treatment. Levels of breakpoint t(12;16) and TERT C228T ctDNA correlated with the clinical course and tumor burden in patients with myxoid liposarcomas (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009= 4). ctDNA could detect minimal residual disease and tumor recurrence. In contrast, detection of MDM2 amplifications was not sensitive enough to detect tumors in patients with well\u2010differentiated\/de\u2010differentiated liposarcomas (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009= 5). Genotyping of cfDNA for tumor specific genetic alterations is a feasible and promising approach for monitoring tumor activity in patients with myxoid liposarcomas. Detection of ctDNA during follow\u2010up examinations despite negative standard imaging studies might warrant more sensitive imaging (e.g. PET\u2010CT) or closer follow\u2010up intervals to timely localize and treat recurrences.\n                  <\/jats:p>","DOI":"10.1002\/ijc.32216","type":"journal-article","created":{"date-parts":[[2019,2,19]],"date-time":"2019-02-19T01:49:26Z","timestamp":1550540966000},"page":"1148-1161","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Genotyping of circulating cell\u2010free DNA enables noninvasive tumor detection in myxoid liposarcomas"],"prefix":"10.1002","volume":"145","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1267-0840","authenticated-orcid":false,"given":"David","family":"Braig","sequence":"first","affiliation":[{"name":"Department of Plastic and Hand Surgery Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg  Freiburg Germany"},{"name":"Plastic and Reconstructive Surgery Sir Charles Gairdner Hospital  Perth, WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Becherer","sequence":"additional","affiliation":[{"name":"Department of Plastic and Hand Surgery Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiane","family":"Bickert","sequence":"additional","affiliation":[{"name":"Department of Plastic and Hand Surgery Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moritz","family":"Braig","sequence":"additional","affiliation":[{"name":"Department of Radiology, Medical Physics Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rainer","family":"Claus","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Stem Cell Transplantation Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg  Freiburg Germany"},{"name":"Department of Internal Medicine Augsburg Hospital, Medical Faculty of the University of Augsburg  Augsburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja E.","family":"Eisenhardt","sequence":"additional","affiliation":[{"name":"Department of Plastic and Hand Surgery Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juergen","family":"Heinz","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Stem Cell Transplantation Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jutta","family":"Scholber","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg W.","family":"Herget","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics and Traumatology Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Bronsert","sequence":"additional","affiliation":[{"name":"Institute for Surgical Pathology Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg  Freiburg Germany"},{"name":"Tumorbank Comprehensive Cancer Center Freiburg Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"Fricke","sequence":"additional","affiliation":[{"name":"Department of Plastic and Hand Surgery Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg  Freiburg Germany"},{"name":"Department of Plastic Aesthetic and Hand Surgery HELIOS Klinikum Emil von Behring  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Follo","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Stem Cell Transplantation Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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