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Additionally, whole genome sequencing (WGS) was compared to WES to determine if WGS would further inform treatment decisions, and whether circulating tumor DNA (ctDNA) could detect the H3K27M mutation to allow assessment of therapy response. Patients were selected across three Pacific Pediatric Neuro\u2010Oncology Consortium member institutions between September 2014 and January 2016. WES and RNAseq were performed at diagnosis and recurrence when possible in a CLIA\u2010certified laboratory. Patient\u2010derived cell line development was attempted for each subject. Collection of blood for ctDNA was done prior to treatment and with each MRI. A specialized tumor board generated a treatment recommendation including up to four FDA\u2010approved agents based upon the genomic alterations detected. A treatment plan was successfully issued within 21 business days from tissue collection for all 15 subjects, with 14 of the 15 subjects fulfilling the feasibility criteria. WGS results did not significantly deviate from WES\u2010based therapy recommendations; however, WGS data provided further insight into tumor evolution and fidelity of patient\u2010derived cell models. Detection of the\n                    <jats:italic>H3F3A<\/jats:italic>\n                    or\n                    <jats:italic>HIST1H3B<\/jats:italic>\n                    K27M (H3K27M) mutation using ctDNA was successful in 92% of H3K27M mutant cases. A personalized treatment recommendation for DIPG can be rendered within a multicenter setting using comprehensive next\u2010generation sequencing technology in a clinically relevant timeframe.\n                  <\/jats:p>","DOI":"10.1002\/ijc.32258","type":"journal-article","created":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T16:36:06Z","timestamp":1552408566000},"page":"1889-1901","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":110,"title":["A pilot precision medicine trial for children with diffuse intrinsic pontine glioma\u2014PNOC003: A report from the Pacific Pediatric Neuro\u2010Oncology Consortium"],"prefix":"10.1002","volume":"145","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3452-5150","authenticated-orcid":false,"given":"Sabine","family":"Mueller","sequence":"first","affiliation":[{"name":"Department of Neurology University of California San Francisco  San Francisco CA USA"},{"name":"Department of 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