{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T22:34:23Z","timestamp":1792276463882,"version":"4.4.0"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T00:00:00Z","timestamp":1567987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["Valid\u2010screen 666800"],"award-info":[{"award-number":["Valid\u2010screen 666800"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2020,8,15]]},"abstract":"<jats:p>\n                    Widespread adoption of primary human papillomavirus (HPV)\u2010based screening has encouraged the search for a triage test which retains high sensitivity for the detection of cervical cancer and precancer, but increases specificity to avoid overtreatment. Methylation analysis of\n                    <jats:italic>FAM19A4<\/jats:italic>\n                    and\n                    <jats:italic>miR124\u20102<\/jats:italic>\n                    genes has shown promise for the triage of high\u2010risk (hr) HPV\u2010positive women. In our study, we assessed the consistency of\n                    <jats:italic>FAM19A4<\/jats:italic>\n                    \/\n                    <jats:italic>miR124\u20102<\/jats:italic>\n                    methylation analysis in the detection of cervical cancer in a series of 519 invasive cervical carcinomas (\n                    <jats:italic>n<\/jats:italic>\n                    = 314 cervical scrapes,\n                    <jats:italic>n<\/jats:italic>\n                    = 205 tissue specimens) from over 25 countries, using a quantitative methylation\u2010specific PCR (qMSP)\u2010based assay (QIAsure Methylation Test\u00ae). Positivity rates stratified per histotype, FIGO stage, hrHPV status, hrHPV genotype, sample type and geographical region were calculated. In total, 510 of the 519 cervical carcinomas (98.3%; 95% CI: 96.7\u201399.2) tested\n                    <jats:italic>FAM19A4\/miR124\u20102<\/jats:italic>\n                    methylation\u2010positive. Test positivity was consistent across the different subgroups based on cervical cancer histotype, FIGO stage, hrHPV status, hrHPV genotype, sample type and geographical region. In conclusion,\n                    <jats:italic>FAM19A4\/miR124\u20102<\/jats:italic>\n                    methylation analysis detects nearly all cervical carcinomas, including rare histotypes and hrHPV\u2010negative carcinomas. These results indicate that a negative\n                    <jats:italic>FAM19A4\/miR124\u20102<\/jats:italic>\n                    methylation assay result is likely to rule out the presence of cervical cancer.\n                  <\/jats:p>","DOI":"10.1002\/ijc.32614","type":"journal-article","created":{"date-parts":[[2019,8,7]],"date-time":"2019-08-07T11:58:14Z","timestamp":1565179094000},"page":"1215-1221","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":81,"title":["<i>FAM19A4\/miR124\u20102<\/i>\n                    methylation in invasive cervical cancer: A retrospective cross\u2010sectional worldwide study"],"prefix":"10.1002","volume":"147","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4951-9167","authenticated-orcid":false,"given":"Frederique J.","family":"Vink","sequence":"first","affiliation":[{"name":"Cancer Center Amsterdam Amsterdam UMC, Vrije Universiteit Amsterdam, Pathology  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris J.L.M.","family":"Meijer","sequence":"additional","affiliation":[{"name":"Cancer Center Amsterdam Amsterdam UMC, Vrije Universiteit Amsterdam, Pathology  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7534-333X","authenticated-orcid":false,"given":"Gary M.","family":"Clifford","sequence":"additional","affiliation":[{"name":"International Agency for Research on Cancer  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Poljak","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"O\u0161trbenk","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology University of Ljubljana  Ljubljana 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Laboratory  Rijswijk The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2604-3928","authenticated-orcid":false,"given":"Kate","family":"Cuschieri","sequence":"additional","affiliation":[{"name":"Scottish HPV Reference Laboratory Royal Infirmary of Edinburgh  Edinburgh United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elia Alca\u00f1iz","family":"Boada","sequence":"additional","affiliation":[{"name":"HPV Research Group, Division of Pathology University of Edinburgh  Edinburgh United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nienke E.","family":"van Trommel","sequence":"additional","affiliation":[{"name":"Department of Gynaecologic Oncology Centre of Gynaecologic Oncology Amsterdam, Antoni van Leeuwenhoek\/Netherlands Cancer Institute  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit 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