{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T09:29:28Z","timestamp":1761989368953,"version":"3.40.5"},"reference-count":27,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,10,15]],"date-time":"2019-10-15T00:00:00Z","timestamp":1571097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:p>Aim of this study was to compare the 5\u2010year risk of cervical intraepithelial neoplasia grade 2+ (CIN2+)\/CIN3+ and the performance parameters at 3\u2010year rescreening of a negative E6\/E7 mRNA\u2010human papillomavirus (HPV) test with those of a HPV\u2010DNA\u2010negative test. We studied a cohort of HPV\u2010negative women tested with the Aptima HPV\u2010mRNA Assay (\u201cHPV\u2010mRNA cohort\u201d) versus a cohort of HPV negatives tested with the Hybrid Capture 2 (HC2) DNA test living in neighboring areas. Both cohorts were rescreened after 3\u2009years by a HPV\u2010DNA test (HC2 or Cobas 4800 HPV test). HPV test positivity, referral to colposcopy and detection of CIN2+ at 3\u2010year rescreening were computed. The Veneto Cancer Registry was checked to search for invasive cancers and CIN3 diagnosed up to 5\u2009years from the negative baseline test. Some 22,338 HPV\u2010mRNA and 68,695 HPV\u2010DNA\u2010negative women were invited to 3\u2010year rescreening, and, respectively, 16,641 (74.5%) and 54,630 (79.6%) complied with the invitation. The proportion of HPV\u2010positive tests, referral to colposcopy and detection of CIN2+ in the HPV\u2010mRNA and HPV\u2010DNA cohorts were, respectively. 4.0 and 3.9% (ratio 1.08; 95% confidence interval [CI] 0.99\u20131.17), 2.6 and 2.5% (ratio 1.06, 95% CI 0.95\u20131.18) and 1.4 and 1.7\u2030 (ratio 0.85, 95% CI 0.54\u20131.33). The relative 5\u2010year cumulative risk of cancer and of CIN2+ in the HPV\u2010mRNA and HPV\u2010DNA cohorts were 4.5 and 8.7\/100,000 (ratio 0.51; 95%CI 0.01\u20134.22) and 1.1 and 1.5\/1,000 (ratio 0.74; 95%CI 0.45\u20131.16), respectively. A negative HPV\u2010mRNA test confers a risk of invasive cervical carcinoma and of CIN2+ at 5\u2009years comparable to that of a negative HPV\u2010DNA test.<\/jats:p>","DOI":"10.1002\/ijc.32695","type":"journal-article","created":{"date-parts":[[2019,10,4]],"date-time":"2019-10-04T03:02:01Z","timestamp":1570158121000},"page":"3114-3123","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Risk of CIN2 or more severe lesions after negative HPV\u2010mRNA E6\/E7 overexpression assay and after negative HPV\u2010DNA test: Concurrent cohorts with a 5\u2010year follow\u2010up"],"prefix":"10.1002","volume":"146","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6025-5214","authenticated-orcid":false,"given":"Manuel","family":"Zorzi","sequence":"first","affiliation":[{"name":"Veneto Tumour Registry, Azienda Zero  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annarosa","family":"Del Mistro","sequence":"additional","affiliation":[{"name":"Immunology and Diagnostic Molecular Oncology Unit Veneto Institute of Oncology IOV\u2010IRCCS  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Giorgi Rossi","sequence":"additional","affiliation":[{"name":"Epidemiology Unit Azienda Unit\u00e0 Sanitaria Locale, IRCCS di Reggio Emilia  Reggio Emilia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Licia","family":"Laurino","sequence":"additional","affiliation":[{"name":"Pathology Unit Dell'Angelo General Hospital  Venice Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Battagello","sequence":"additional","affiliation":[{"name":"Veneto Tumour Registry, Azienda Zero  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melania","family":"Lorio","sequence":"additional","affiliation":[{"name":"Department of Prevention Local Health Authority 3 Serenissima  Venice Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marika","family":"Sold\u00e0","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Dell'Angelo General Hospital  Venice Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Martinotti Gabellotti","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Dell'Angelo General Hospital  Venice Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michela","family":"Maran","sequence":"additional","affiliation":[{"name":"Pathology Unit Dell'Angelo General Hospital  Venice Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonella","family":"Dal Cin","sequence":"additional","affiliation":[{"name":"Veneto Tumour Registry, Azienda Zero  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annarita","family":"Fiore","sequence":"additional","affiliation":[{"name":"Veneto Tumour Registry, Azienda Zero  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massimo","family":"Rugge","sequence":"additional","affiliation":[{"name":"Veneto Tumour Registry, Azienda Zero  Padua Italy"},{"name":"Deparment of Diagnostic Medical Sciences and Special Therapies University of Padova  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiziano","family":"Maggino","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Dell'Angelo General Hospital  Venice Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,10,15]]},"reference":[{"volume-title":"WHO guidelines for screening and treatment of precancerous lesions for cervical cancer prevention","year":"2013","author":"World 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