{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T15:19:36Z","timestamp":1790435976107,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002920","name":"Research Grants Council, University Grants Committee","doi-asserted-by":"publisher","award":["17101715"],"award-info":[{"award-number":["17101715"]}],"id":[{"id":"10.13039\/501100002920","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2020,5,15]]},"abstract":"<jats:p><jats:italic>Mirror Image Polydactyly 1 (MIPOL1)<\/jats:italic> is generally associated with congenital anomalies. However, its role in cancer development is poorly understood. Previously, by utilizing the functional complementation approach, microcell\u2010mediated chromosome transfer (MMCT), a tumor suppressor gene, <jats:italic>MIPOL1<\/jats:italic>, was identified. <jats:italic>MIPOL1<\/jats:italic> was confirmed to be downregulated in nasopharyngeal carcinoma (NPC) cells and tumor tissues, and re\u2010expression of <jats:italic>MIPOL1<\/jats:italic> induced tumor suppression. The aim of the current study is to further elucidate the functional tumor suppressive role of <jats:italic>MIPOL1<\/jats:italic>. In our study, with an expanded sample size of different clinical stages of NPC tumor tissues, we further confirmed the downregulation of <jats:italic>MIPOL1<\/jats:italic> in different cancer stages. <jats:italic>MIPOL1<\/jats:italic> re\u2010expression down\u2010regulated angiogenic factors and reduced phosphorylation of metastasis\u2010associated proteins including AKT, p65, and FAK. In addition, MIPOL1 was confirmed to interact with a tumor suppressor, RhoB, and re\u2010expression of MIPOL1 enhanced RhoB activity. The functional role of MIPOL1 was further validated by utilizing a panel of wild\u2010type (WT) and truncated <jats:italic>MIPOL1<\/jats:italic> expression constructs. The <jats:italic>MIPOL1<\/jats:italic> tumor\u2010suppressive effect can only be observed in the WT <jats:italic>MIPOL1\u2010<\/jats:italic>expressing cells. <jats:italic>In vitro<\/jats:italic> and nude mice <jats:italic>in vivo<\/jats:italic> functional studies further confirmed the critical role of WT <jats:italic>MIPOL1<\/jats:italic> in inhibiting migration, invasion and metastasis in NPC. Overall, our study provides strong evidence about the tumor\u2010suppressive role of <jats:italic>MIPOL1<\/jats:italic> in inhibiting angiogenesis and metastasis in NPC.<\/jats:p>","DOI":"10.1002\/ijc.32732","type":"journal-article","created":{"date-parts":[[2019,10,14]],"date-time":"2019-10-14T15:40:05Z","timestamp":1571067605000},"page":"2891-2900","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Functional characterization of a candidate tumor suppressor gene, <i>Mirror Image Polydactyly 1<\/i>, in nasopharyngeal carcinoma"],"prefix":"10.1002","volume":"146","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4753-2285","authenticated-orcid":false,"given":"Merrin M.L.","family":"Leong","sequence":"first","affiliation":[{"name":"Department of Clinical Oncology University of Hong Kong  Pok Fu Lam Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur 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