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Triage tests that improve specificity but maintain high sensitivity are needed. Women enrolled in the experimental arm of Phase 2 of the New Technologies for Cervical Cancer randomized controlled cervical screening trial were tested for high\u2010risk HPV (hrHPV) and referred to colposcopy if positive. hrHPV\u2010positive women also had HPV genotyping (by polymerase chain reaction with GP5+\/GP6+ primers and reverse line blotting), immunostaining for p16 overexpression and cytology. We computed sensitivity, specificity and positive predictive value (PPV) for different combinations of tests and determined potential hierarchical ordering of triage tests. A number of 1,091 HPV\u2010positive women had valid tests for cytology, p16 and genotyping. Ninety\u2010two of them had cervical intraepithelial neoplasia grade 2+ (CIN2+) histology and 40 of them had CIN grade 3+ (CIN3+) histology. The PPV for CIN2+ was &gt;10% in hrHPV\u2010positive women with positive high\u2010grade squamous intraepithelial lesion (61.3%), positive low\u2010grade squamous intraepithelial lesion (LSIL+) (18.3%) and positive atypical squamous cells of undetermined significance (14.8%) cytology, p16 positive (16.7%) and, hierarchically, for infections by HPV33, 16, 35, 59, 31 and 52 (in decreasing order). Referral of women positive for either p16 or LSIL+ cytology had 97.8% sensitivity for CIN2+ and women negative for both of these had a 3\u2010year CIN3+ risk of 0.2%. Similar results were seen for women being either p16 or HPV16\/33 positive. hrHPV\u2010positive women who were negative for p16 and cytology (LSIL threshold) had a very low CIN3+ rate in the following 3\u2009years. Recalling them after that interval and referring those positive for either test to immediate colposcopy seem to be an efficient triage strategy. The same applies to p16 and HPV16.<\/jats:p>","DOI":"10.1002\/ijc.32973","type":"journal-article","created":{"date-parts":[[2020,3,14]],"date-time":"2020-03-14T13:38:46Z","timestamp":1584193126000},"page":"1864-1873","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Combined use of cytology, p16 immunostaining and genotyping for triage of women positive for high\u2010risk human papillomavirus at primary screening"],"prefix":"10.1002","volume":"147","author":[{"given":"Jack","family":"Cuzick","sequence":"first","affiliation":[{"name":"Wolfson Institute of Preventive Medicine, Queen Mary University of London  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2856-7108","authenticated-orcid":false,"given":"Rachael","family":"Adcock","sequence":"additional","affiliation":[{"name":"Wolfson Institute of Preventive Medicine, Queen Mary University of London  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Carozzi","sequence":"additional","affiliation":[{"name":"Regional Cancer Prevention Laboratory, Oncological Network, Prevention and Research Institute (ISPRO)  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Gillio\u2010Tos","sequence":"additional","affiliation":[{"name":"Cancer Epidemiology Unit, Department of Medical Sciences C.E.R.M.S, University of Turin  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"De Marco","sequence":"additional","affiliation":[{"name":"Cancer Epidemiology Unit, Department of Medical Sciences C.E.R.M.S, University of Turin  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annarosa","family":"Del Mistro","sequence":"additional","affiliation":[{"name":"Immunology and Diagnostic Molecular Oncology Unit Veneto Institute of Oncology IOV IRCCS  Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Frayle","sequence":"additional","affiliation":[{"name":"Immunology and Diagnostic Molecular Oncology Unit Veneto Institute of Oncology IOV IRCCS  Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Girlando","sequence":"additional","affiliation":[{"name":"Pathology Unit, S. 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