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The yCD::UPRT\u2010MSC exosomes are internalized by tumor cells and intracellularly convert prodrug 5\u2010fluorocytosine (5\u2010FC) to cytotoxic drug 5\u2010fluorouracil (5\u2010FU). Human tumor cells with the potential to metastasize release exosomes involved in the creation of a premetastatic niche at the predicted organs. We found that cancer cells stably transduced with yCD::UPRT gene by retrovirus infection released exosomes acting similarly like yCD::UPRT\u2010MSC exosomes. Different types of tumor cells were transduced with the yCD::UPRT gene. The homogenous cell population of yCD::UPRT\u2010transduced tumor cells expressed the yCD::UPRT suicide gene and secreted continuously exosomes with suicide gene mRNA in their cargo. All tumor cell suicide gene exosomes upon internalization into the recipient tumor cells induced the cell death by intracellular conversion of 5\u2010FC to 5\u2010FU and to 5\u2010FUMP in a dose\u2010dependent manner. Most of tumor cell\u2010derived suicide gene exosomes were tumor tropic, in 5\u2010FC presence they killed tumor cells but did not inhibit the growth of human skin fibroblast as well as DP\u2010MSCs. Tumor cell\u2010derived suicide gene exosomes home to their cells of origin and hold an exciting potential to become innovative specific therapy for tumors and potentially for metastases.<\/jats:p>","DOI":"10.1002\/ijc.33188","type":"journal-article","created":{"date-parts":[[2020,7,4]],"date-time":"2020-07-04T18:46:11Z","timestamp":1593888371000},"page":"128-139","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Intracellular prodrug gene therapy for cancer mediated by tumor cell suicide gene exosomes"],"prefix":"10.1002","volume":"148","author":[{"given":"Ursula","family":"Altanerova","sequence":"first","affiliation":[{"name":"Department of Stem Cell Preparation St. Elisabeth Cancer Institute  Bratislava Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jana","family":"Jakubechova","sequence":"additional","affiliation":[{"name":"Department of Stem Cell Preparation St. Elisabeth Cancer 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