{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:27:29Z","timestamp":1792715249540,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,8,24]],"date-time":"2020-08-24T00:00:00Z","timestamp":1598227200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["FDN\u2010143347"],"award-info":[{"award-number":["FDN\u2010143347"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2021,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We compared clinical performance of p16\/Ki\u201067 dual\u2010stained cytology and human papillomavirus (HPV) genotyping, via different algorithms\u2014alone, or in combination with cytology\u2014to identify cervical intraepithelial neoplasia grade 2 or worse (CIN2+) and grade 3 or worse (CIN3+) in women referred to as colposcopy. We included 492 cervical specimens (134 normal, 130 CIN1, 99 CIN2, 121 CIN3, 8 cancers) randomly selected from 1158 specimens with valid conventional cytology, HPV (cobas 4800 HPV test) and biopsy results. Dual\u2010stained cytology was retrospectively performed (CINtec PLUS assay) on PreservCyt material; slides were read by a cytologist and confirmed by two pathologists, blinded to cytology, biopsy and genotyping results. Sensitivity and specificity (95% confidence intervals in parentheses) of dual\u2010stained cytology to detect CIN2+ and CIN3+ were compared to other screening tests available for the same women. Positivity rate for dual\u2010stained cytology increased with histological severity: 30.6% in normal, 41.5% in CIN1, 72.7% in CIN2, 86.8% in CIN3 and 87.5% in cancer. Dual\u2010stained cytology alone had lower sensitivity than HPV testing for CIN2+ [80.7% (75.0\u201085.6) vs 89.9% (85.3\u201093.5)] and CIN3+ [86.8% (79.7\u201092.1) vs 92.3% (86.2\u201096.2)]. However, corresponding specificity values were higher [64.0% (57.9\u201069.8) vs 56.1% (49.8\u201062.1) for CIN2+; 54.0% (48.7\u201059.2) vs 44.4% (39.2\u201049.6) for CIN3+]. Combining dual\u2010stained cytology with an ASC\u2010US abnormality threshold decreased specificity to 31.4% (25.9\u201037.4) for CIN2+ and 24.2% (19.9\u201029.0) for CIN3+. The corresponding values considering low squamous intraepithelial lesion threshold values were 42.8% (36.8\u201049.0) and 35.0% (30.1\u201040.1). Dual\u2010stained cytology and HPV testing exhibited similar performance, although the former improved the specificity by 7.9% and 9.6% for CIN2+ and CIN3+, respectively.<\/jats:p>","DOI":"10.1002\/ijc.33250","type":"journal-article","created":{"date-parts":[[2020,8,12]],"date-time":"2020-08-12T03:43:00Z","timestamp":1597203780000},"page":"492-501","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Dual staining for p16\/Ki\u201067 to detect high\u2010grade cervical lesions: Results from the Screening Triage Ascertaining Intraepithelial Neoplasia by Immunostain Testing study"],"prefix":"10.1002","volume":"148","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5190-0370","authenticated-orcid":false,"given":"Mariam","family":"El\u2010Zein","sequence":"first","affiliation":[{"name":"Division of Cancer Epidemiology McGill University  Montr\u00e9al Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter","family":"Gotlieb","sequence":"additional","affiliation":[{"name":"Division of Gynecologic Oncology and Colposcopy McGill University\u2014Jewish General Hospital  Montr\u00e9al Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy","family":"Gilbert","sequence":"additional","affiliation":[{"name":"Gynecologic Cancer Service McGill University Health Centre\u2014Glen Site Cedars Cancer Centre  Montr\u00e9al Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Hemmings","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology McGill University Health Centre\u2014St Mary's Hospital Centre  Montr\u00e9al Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel A.","family":"Behr","sequence":"additional","affiliation":[{"name":"Department of Medicine McGill University Health Centre  Montr\u00e9al Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4409-8084","authenticated-orcid":false,"given":"Eduardo L.","family":"Franco","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology McGill University  Montr\u00e9al Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"for the STAIN\u2010IT Study 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