{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T15:53:18Z","timestamp":1779205998533,"version":"3.51.4"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T00:00:00Z","timestamp":1599782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP16K09295"],"award-info":[{"award-number":["JP16K09295"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP19H03521"],"award-info":[{"award-number":["JP19H03521"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic>) are a primary factor in the pathogenesis of gastric cancer (GC); GC ranks third among cancer\u2010related mortality. A clear understanding of the <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> genome factors underlying GC is necessary to develop more effective methods to prevent GC. A single\u2010molecule real\u2010time DNA sequencing\u2010based <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> genome\u2010wide association study analysis was performed using the <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> genome present in five early\u2010stage GC (EGC) and five non\u2010GC clinical DNA samples recovered from gastric washes. A total of 275 genes with 702 nucleotide variants (NVs) were found to be common to three or more patients with EGC but no non\u2010GC patients (single\u2010NV: 654\/702, 93.2%; multi\u2010NV: 40\/702, 5.7%; deletion: 3\/702, 0.4%; insertion: 3\/702, 0.7%). Gene ontology analysis of <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> revealed that genes involved in the mitochondrial electron transport system, glycolytic processes and the TCA cycle were highly enriched. Cancer\u2010related NVs were most frequently found in a member of the <jats:italic>Helicobacter<\/jats:italic> outer membrane protein family, <jats:italic>hopL<\/jats:italic>. In particular, one of the NVs in <jats:italic>hopL<\/jats:italic> was a novel six\u2010nucleotide insertion (1159095\u03021159096, TACTTC); this mutant was detected more frequently in a validation set of 50 additional EGC samples (22\/50, 44.0%) than in 18 non\u2010GC samples (3\/18, 16.7%, <jats:italic>P<\/jats:italic> = .04). These results suggest that the <jats:italic>hopL<\/jats:italic> variant is associated with the development of GC and may serve as a genetic biomarker of <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> virulence and GC risk. Our assay can serve as a potent tool to expand our understanding of bacteria\u2010associated tumorigenesis.<\/jats:p>","DOI":"10.1002\/ijc.33257","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T13:22:51Z","timestamp":1598620971000},"page":"178-192","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Cancer\u2010related genetic variants of <i>Helicobacter pylori<\/i> strains determined using gastric wash\u2010based whole\u2010genome analysis with single\u2010molecule real\u2010time technology"],"prefix":"10.1002","volume":"148","author":[{"given":"Yoshiyuki","family":"Watanabe","sequence":"first","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Internal Medicine St. Marianna University School of Medicine  Kawasaki Japan"},{"name":"Department of Internal Medicine Kawasaki Rinko General Hospital  Kawasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ritsuko","family":"Oikawa","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Internal Medicine St. Marianna University School of Medicine  Kawasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiro","family":"Kodaka","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology Musashikosugi Hospital, Nippon Medical School  Kawasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinori","family":"Sato","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Internal Medicine St. Marianna University School of Medicine  Kawasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoko","family":"Ono","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Hokkaido University Hospital  Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Kenmochi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Saiseikai Kanagawa Hospital  Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideo","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Institute of Clinical Medicine, University of Tsukuba  Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiji","family":"Futagami","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology Musashikosugi Hospital, Nippon Medical School  Kawasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mototsugu","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology National Hospital Organization Hakodate Hospital  Hakodate Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0954-6806","authenticated-orcid":false,"given":"Hiroyuki","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Internal Medicine St. Marianna University School of Medicine  Kawasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumio","family":"Itoh","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Internal Medicine St. Marianna University School of Medicine  Kawasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2017.04.022"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/340157"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1001110"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1708423"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2017-313863"},{"key":"e_1_2_10_7_1","doi-asserted-by":"crossref","first-page":"e00286\u201014","DOI":"10.1128\/genomeA.00286-14","article-title":"Complete genome sequences of eight Helicobacter pylori strains with different virulence factor genotypes and methylation profiles, isolated from patients with diverse gastrointestinal diseases on Okinawa Island, Japan, determined using PacBio single\u2010molecule real\u2010time technology","volume":"2","author":"Satou K","year":"2014","journal-title":"Genome Announc"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00239-11"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01021-13"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1757-4749-6-27"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0067539"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13099-017-0165-1"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2017.10.014"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10050-1"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.251551698"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.12.7832-7838.2001"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1198-743x.2001.00226.x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.02.085"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000332570"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ctg.2016.40"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-016-4886-4"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/1010428317734865"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.175240.114"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2010.154"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/371503"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2012.00046"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins9030101"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2017.06.004"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.33257","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ijc.33257","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.33257","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T11:46:47Z","timestamp":1693828007000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.33257"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,11]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1002\/ijc.33257"],"URL":"https:\/\/doi.org\/10.1002\/ijc.33257","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,11]]},"assertion":[{"value":"2020-04-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}