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To develop new in vitro and in vivo models of ACC, the techniques of organoid culture and patient\u2010derived tumor xenograft (PDX), which have attracted attention in other malignancies in recent years, were applied. Tumor specimens from surgically resected salivary ACC were proceeded for the preparation of PDX and organoid culture. The orthotopic transplantation of patient\u2010derived or PDX\u2010derived organoids was demonstrated into submandibular glands of NSG mice and those histology was evaluated. PDX\u2010derived organoid cells were evaluated for the presence of <jats:italic>MYB<\/jats:italic>\u2010mediated fusion genes and proceeded for in vitro drug sensitivity assay. Human ACC\u2010derived organoids were successfully generated in three\u2010dimensional culture and confirmed the ability of these cells to form tumors by orthotopic injection. Short\u2010term organoid cell cultures from two individual ACC PDX tumors were also established that maintain the characteristic <jats:italic>MYBL1<\/jats:italic> translocation and histological features of the original parent and PDX tumors. Finally, the establishment of drug sensitivity tests on these short\u2010term cultured cells was confirmed using three different agents. This is the first to report an approach for the generation of human ACC\u2010derived organoids as in vitro and in vivo cancer models, providing insights into understanding of the ACC biology and creating personalized therapy design for patients with ACC.<\/jats:p>","DOI":"10.1002\/ijc.33315","type":"journal-article","created":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T06:46:21Z","timestamp":1601275581000},"page":"193-202","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Establishment of <scp>PDX<\/scp>\u2010derived salivary adenoid cystic carcinoma cell lines using organoid culture method"],"prefix":"10.1002","volume":"148","author":[{"given":"Kentaro","family":"Takada","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery Yokohama City University, School of Medicine  Yokohama 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