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NPAs may also promote disease worsening in various inflammatory diseases. However, characterization of NPAs in cancer remains totally unexplored. Using ImageStreamX (ISX) imaging flow cytometer, we were not only allowed able to detect CD15\n                    <jats:sup>+<\/jats:sup>\n                    CD14\n                    <jats:sup>\u2212<\/jats:sup>\n                    CD36\n                    <jats:sup>+<\/jats:sup>\n                    ITGA2B\n                    <jats:sup>+<\/jats:sup>\n                    NPAs in both healthy donors' (HDs) and cancer patients' bloods, but we also showed that NPAs result from the binding of platelets preferentially to low\u2010density neutrophils (LDNs) as opposed to normal\u2010density neutrophils (NDNs). By reanalyzing two independent public scRNAseq data of whole blood leukocytes from cancer patients and HDs, we could identify a subset of neutrophils with high platelet gene expression that may correspond to NPAs. Moreover, we showed that cancer patients' derived NPAs possessed a distinct molecular signature compared to the other neutrophil subsets, independently of platelet genes. Gene ontology (GO) term enrichment analysis of this NPAs\u2010associated neutrophil transcriptomic signature revealed a significant enrichment of neutrophil degranulation, chemotaxis and trans\u2010endothelial migration GO terms. Lastly, using The Cancer Genome Atlas (TCGA), we could show by multivariate Cox analysis that the NPAs\u2010associated neutrophil transcriptomic signature was associated with a worse patient prognosis in several cancer types. These results suggest that neutrophils from NPAs are systemically primed by platelets empowering them with cancer progression capacities once at tumor site. NPAs may therefore hold clinical utility as novel noninvasive blood prognostic biomarker in cancer patients with solid tumors.\n                  <\/jats:p>","DOI":"10.1002\/ijc.33991","type":"journal-article","created":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T05:04:52Z","timestamp":1646629492000},"page":"138-152","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Gene signature of circulating platelet\u2010bound neutrophils is associated with poor prognosis in cancer patients"],"prefix":"10.1002","volume":"151","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6338-8784","authenticated-orcid":false,"given":"Pac\u00f4me","family":"Lecot","sequence":"first","affiliation":[{"name":"TERI (Tumor Escape, Resistance and Immunity) Department, Centre de Recherche en Canc\u00e9rologie de Lyon, Centre L\u00e9on B\u00e9rard Universit\u00e9 de Lyon, Universit\u00e9 Claude Bernard Lyon 1, INSERM 1052, CNRS 5286  Lyon 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