{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T01:03:35Z","timestamp":1790730215116,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T00:00:00Z","timestamp":1647388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006188","name":"Einstein Stiftung Berlin","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012353","name":"Deutschen Konsortium f\u00fcr Translationale Krebsforschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012353","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017268","name":"Berlin Institute of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100017268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2022,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Lung carcinoid tumors, also referred to as pulmonary neuroendocrine tumors or lung carcinoids, are rare neoplasms of the lung with a more favorable prognosis than other subtypes of lung cancer. Still, some patients suffer from relapsed disease and metastatic spread. Several recent single\u2010cell studies have provided detailed insights into the cellular heterogeneity of more common lung cancers, such as adeno\u2010 and squamous cell carcinoma. However, the characteristics of lung carcinoids on the single\u2010cell level are yet completely unknown. To study the cellular composition and single\u2010cell gene expression profiles in lung carcinoids, we applied single\u2010cell RNA sequencing to three lung carcinoid tumor samples and normal lung tissue. The single\u2010cell transcriptomes of carcinoid tumor cells reflected intertumoral heterogeneity associated with clinicopathological features, such as tumor necrosis and proliferation index. The immune microenvironment was specifically enriched in noninflammatory monocyte\u2010derived myeloid cells. Tumor\u2010associated endothelial cells were characterized by distinct gene expression profiles. A spectrum of vascular smooth muscle cells and pericytes predominated the stromal microenvironment. We found a small proportion of myofibroblasts exhibiting features reminiscent of cancer\u2010associated fibroblasts. Stromal and immune cells exhibited potential paracrine interactions which may shape the microenvironment via NOTCH, VEGF, TGF\u03b2 and JAK\/STAT signaling. Moreover, single\u2010cell gene signatures of pericytes and myofibroblasts demonstrated prognostic value in bulk gene expression data. Here, we provide first comprehensive insights into the cellular composition and single\u2010cell gene expression profiles in lung carcinoids, demonstrating the noninflammatory and vessel\u2010rich nature of their tumor microenvironment, and outlining relevant intercellular interactions which could serve as future therapeutic targets.<\/jats:p>","DOI":"10.1002\/ijc.33995","type":"journal-article","created":{"date-parts":[[2022,3,9]],"date-time":"2022-03-09T08:17:46Z","timestamp":1646813866000},"page":"2058-2071","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["The single\u2010cell transcriptional landscape of lung carcinoid tumors"],"prefix":"10.1002","volume":"150","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4442-7116","authenticated-orcid":false,"given":"Philip","family":"Bischoff","sequence":"first","affiliation":[{"name":"Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie 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