{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T13:32:59Z","timestamp":1793712779670,"version":"4.6.1"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,6,24]],"date-time":"2022-06-24T00:00:00Z","timestamp":1656028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Accelerated cervical cancer control will require widespread human papillomavirus (HPV) vaccination and screening. For screening, sensitive HPV testing with an option of self\u2010collection is increasingly desirable. HPV typing predicts risk of precancer\/cancer, which could be useful in management, but most current typing assays are expensive and\/or complicated. An existing 15\u2010type isothermal amplification assay (AmpFire, Atila Biosystems, USA) was redesigned as a 13\u2010type assay (ScreenFire) for public health use. The redesigned assay groups HPV types into four channels with differential cervical cancer risk: (a) HPV16, (b) HPV18\/45, (c) HPV31\/33\/35\/52\/58 and (d) HPV39\/51\/56\/59\/68. Since the assay will be most useful in resource\u2010limited settings, we chose a stratified random sample of 453 provider\u2010collected samples from a population\u2010based screening study in rural Nigeria that had been initially tested with MY09\u2010MY11\u2010based PCR with oligonucleotide hybridization genotyping. Frozen residual specimens were masked and retested at Atila Biosystems. Agreement on positivity between ScreenFire and prior PCR testing was very high for each of the channels. When we simulated intended use, that is, a hierarchical result in order of clinical importance of the type groups (HPV16 &gt; 18\/45 &gt; 31\/33\/35\/52\/58 &gt; 39\/51\/56\/59\/68), the weighted kappa for ScreenFire vs PCR was 0.90 (95% CI: 0.86\u20100.93). The ScreenFire assay is mobile, relatively simple, rapid (results within 20\u201060\u2009minutes) and agrees well with reference testing particularly for the HPV types of greatest carcinogenic risk. If confirmed, ScreenFire or similar isothermal amplification assays could be useful as part of risk\u2010based screening and management.<\/jats:p>","DOI":"10.1002\/ijc.34151","type":"journal-article","created":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T15:24:54Z","timestamp":1654529094000},"page":"1142-1149","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Redesign of a rapid, low\u2010cost <scp>HPV<\/scp> typing assay to support risk\u2010based cervical screening and management"],"prefix":"10.1002","volume":"151","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8992-5944","authenticated-orcid":false,"given":"Kanan T.","family":"Desai","sequence":"first","affiliation":[{"name":"Clinical Epidemiology Unit, Clinical Genetic Branch, Division of Cancer Epidemiology and Genetics National Cancer Institute  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clement A.","family":"Adepiti","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Perinatology The Obafemi Awolowo University  Ile\u2010Ife Osun State Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Schiffman","sequence":"additional","affiliation":[{"name":"Clinical Epidemiology Unit, Clinical Genetic Branch, Division of Cancer Epidemiology and Genetics National Cancer Institute  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didem","family":"Egemen","sequence":"additional","affiliation":[{"name":"Clinical Epidemiology Unit, Clinical Genetic Branch, Division of Cancer Epidemiology and Genetics National Cancer Institute  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia C.","family":"Gage","sequence":"additional","affiliation":[{"name":"Clinical Epidemiology Unit, Clinical Genetic Branch, Division of Cancer Epidemiology and Genetics National Cancer Institute  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1251-0836","authenticated-orcid":false,"given":"Nicolas","family":"Wentzensen","sequence":"additional","affiliation":[{"name":"Clinical Epidemiology Unit, Clinical Genetic Branch, Division of Cancer Epidemiology and Genetics National Cancer Institute  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"de Sanjose","sequence":"additional","affiliation":[{"name":"Clinical Epidemiology Unit, Clinical Genetic Branch, Division of Cancer Epidemiology and Genetics National Cancer Institute  Rockville Maryland USA"},{"name":"ISGlobal  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert D.","family":"Burk","sequence":"additional","affiliation":[{"name":"Albert Einstein Cancer Center Albert Einstein College of Medicine  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayode O.","family":"Ajenifuja","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Perinatology The Obafemi Awolowo University  Ile\u2010Ife Osun State Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,6,24]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"International Agency for Research on Cancer.GCO Cancer Today [Internet]. World Health Organization;2021.https:\/\/gco.iarc.fr\/today\/online-analysis-map?v=2020&mode=population&mode_population=continents&population=900&populations=900&key=asr&sex=2&cancer=23&type=1&statistic=5&prevalence=0&population_group=0&ages_group%5B%5D=0&ages_group%5B%5D=17&nb_items=10&gr. Accessed September 28 2021."},{"key":"e_1_2_11_3_1","unstructured":"Cervical Cancer: An NCD We Can Overcome [Internet].https:\/\/www.who.int\/dg\/speeches\/detail\/cervical-cancer-an-ncd-we-can-overcome. 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