{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T18:41:55Z","timestamp":1780339315571,"version":"3.54.1"},"reference-count":27,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,6,20]],"date-time":"2022-06-20T00:00:00Z","timestamp":1655683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pregnant women diagnosed with CIN3 have high regression rates after delivery. Biomarkers are needed to only identify pregnant women with progressive CIN requiring treatment to reduce overreferral and overtreatment. In our study we evaluated the performance of the FAM19A4\/miR124\u20102 methylation test for molecular triage on FFPE samples of CIN3+\u2010diagnosed pregnant women with known clinical course over time as well in a cross\u2010sectional setting. In this German multicenter retrospective study biopsy material was collected from pregnant women diagnosed with cervical cancer (n\u00a0=\u00a016), with CIN3 that progressed to cancer during pregnancy (n\u00a0=\u00a07), with CIN3 that regressed to CIN1 or less within 6\u00a0months after delivery (n\u00a0=\u00a041), without CIN (n\u00a0=\u00a016), CIN3 covering 3\u20104 quadrants (n\u00a0=\u00a014) and randomly selected CIN3 (n\u00a0=\u00a041). <jats:italic>FAM19A4\/miR124\u20102<\/jats:italic> methylation analysis was performed blinded on first diagnosis. All pregnant women with cervical cancer and with CIN3 progressing to cancer tested positive for <jats:italic>FAM19A4\/miR124\u20102<\/jats:italic> methylation (100%, 22\/22). In the regressing CIN3 group 47.5% and in the group without CIN 21.6% tested methylation positive. High\u2010volume CIN3 and random selected CIN3 were methylation\u2010positive in 91.7% and 82.1%, respectively. Methylation levels were significantly higher in progressive CIN3 and cancer compared to the controls (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.0005). The likelihood ratio of a negative methylation test (LR\u2212) for progressive CIN3+ was 0 (95% CI: 0\u20100.208). A negative <jats:italic>FAM19A4\/miR124\u20102<\/jats:italic> methylation test can rule out progressive CIN disease in pregnant women diagnosed with CIN3. This can help the clinician by managing these pregnant women with conservative follow\u2010up until after delivery.<\/jats:p>","DOI":"10.1002\/ijc.34153","type":"journal-article","created":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T15:24:53Z","timestamp":1654529093000},"page":"1578-1585","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Evaluation of <i>FAM19A4\/miR124\u20102<\/i> methylation performance in the management of <scp>CIN3<\/scp> diagnosed pregnant women"],"prefix":"10.1002","volume":"151","author":[{"given":"Monika","family":"Hampl","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology University Medical Center D\u00fcsseldorf  D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4282-4417","authenticated-orcid":false,"given":"Albertus T.","family":"Hesselink","sequence":"additional","affiliation":[{"name":"Self\u2010screen B.V.  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