{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T06:22:05Z","timestamp":1788848525775,"version":"build-2803163510"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T00:00:00Z","timestamp":1680220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR20\u2010CE14\u20100007"],"award-info":[{"award-number":["ANR20\u2010CE14\u20100007"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004097","name":"Fondation ARC pour la Recherche sur le Cancer","doi-asserted-by":"publisher","award":["PJA 20191209289"],"award-info":[{"award-number":["PJA 20191209289"]}],"id":[{"id":"10.13039\/501100004097","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Adrenocortical carcinoma (ACC) is a rare endocrine malignancy with a high risk of relapse and metastatic spread. The actin\u2010bundling protein fascin (FSCN1) is overexpressed in aggressive ACC and represents a reliable prognostic indicator. FSCN1 has been shown to synergize with VAV2, a guanine nucleotide exchange factor for the Rho\/Rac GTPase family, to enhance the invasion properties of ACC cancer cells. Based on those results, we investigated the effects of FSCN1 inactivation by CRISPR\/Cas9 or pharmacological blockade on the invasive properties of ACC cells, both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and in an\n                    <jats:italic>in vivo<\/jats:italic>\n                    metastatic ACC zebrafish model. Here, we showed that\n                    <jats:italic>FSCN1<\/jats:italic>\n                    is a transcriptional target for \u03b2\u2010catenin in H295R ACC cells and that its inactivation resulted in defects in cell attachment and proliferation. FSCN1 knock\u2010out modulated the expression of genes involved in cytoskeleton dynamics and cell adhesion. When Steroidogenic Factor\u20101 (SF\u20101) dosage was upregulated in H295R cells, activating their invasive capacities,\n                    <jats:italic>FSCN1<\/jats:italic>\n                    knock\u2010out reduced the number of filopodia, lamellipodia\/ruffles and focal adhesions, while decreasing cell invasion in Matrigel. Similar effects were produced by the FSCN1 inhibitor G2\u2010044, which also diminished the invasion of other ACC cell lines expressing lower levels of FSCN1 than H295R. In the zebrafish model, metastases formation was significantly reduced in\n                    <jats:italic>FSCN1<\/jats:italic>\n                    knock\u2010out cells and G2\u2010044 significantly reduced the number of metastases formed by ACC cells. Our results indicate that FSCN1 is a new druggable target for ACC and provide the rationale for future clinical trials with FSCN1 inhibitors in patients with ACC.\n                  <\/jats:p>","DOI":"10.1002\/ijc.34526","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T05:39:41Z","timestamp":1679895581000},"page":"210-223","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["<scp>FSCN1<\/scp>\n                    as a new druggable target in adrenocortical carcinoma"],"prefix":"10.1002","volume":"153","author":[{"given":"Carmen","family":"Ruggiero","sequence":"first","affiliation":[{"name":"Institut de Pharmacologie Mol\u00e9culaire et Cellulaire CNRS UMR 7275  06560 Valbonne France"},{"name":"Universit\u00e9 C\u00f4te d'Azur  06560 Valbonne 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06560 Valbonne France"},{"name":"Universit\u00e9 C\u00f4te d'Azur  06560 Valbonne France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Cantini","sequence":"additional","affiliation":[{"name":"Endocrinology Unit, Department of Experimental and Clinical Biomedical Sciences \"Mario Serio\" University of Florence  50134 Florence Italy"},{"name":"Centro di Ricerca e Innovazione sulle Patologie Surrenaliche  AOU Careggi 50134 Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Cioppi","sequence":"additional","affiliation":[{"name":"Centro di Ricerca e Innovazione sulle Patologie Surrenaliche  AOU Careggi 50134 Florence Italy"},{"name":"Department of Experimental and Clinical Medicine University of Florence  50134 Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constanze","family":"Hantel","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Diabetology and Clinical Nutrition University Hospital Zurich (USZ) and University of Zurich (UZH)  8091 Z\u00fcrich Switzerland"},{"name":"Medizinische Klinik und Poliklinik III University Hospital Carl Gustav Carus Dresden  01307 Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Kiseljak\u2010Vassiliades","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Metabolism and Diabetes, Department of Medicine University of Colorado Anschutz Medical Campus  80045 Aurora Colorado USA"},{"name":"Rocky Mountain Regional Veterans Affairs Medical Center  80045 Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret E.","family":"Wierman","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Metabolism and Diabetes, Department of Medicine University of Colorado Anschutz Medical Campus  80045 Aurora Colorado USA"},{"name":"Rocky Mountain Regional Veterans Affairs Medical Center  80045 Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura\u2010Sophie","family":"Landwehr","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Diabetology\u2014Department of Internal Medicine I University Hospital, University of W\u00fcrzburg  97080 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Weigand","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Diabetology\u2014Department of Internal Medicine I University Hospital, University of W\u00fcrzburg  97080 W\u00fcrzburg Germany"},{"name":"Department of Medicine IV University Hospital Munich, Ludwig\u2010Maximilians\u2010Universit\u00e4t M\u00fcnchen  81377 Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Kurlbaum","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Diabetology\u2014Department of Internal Medicine I 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